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Titolo:
Transport of large neutral amino acids into BeWo cells
Autore:
Eaton, BM; Sooranna, SR;
Indirizzi:
Chelsea & Westminster Hosp, Imperial Coll, Sch Med, Dept Maternal & Fetal Med, London SW10 9NH, England Chelsea & Westminster Hosp London England SW10 9NH don SW10 9NH, England
Titolo Testata:
PLACENTA
fascicolo: 5-6, volume: 21, anno: 2000,
pagine: 558 - 564
SICI:
0143-4004(200007/08)21:5-6<558:TOLNAA>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
SUPERFUSED MICROCARRIER CULTURES; SYSTEM Y(+)L; HUMAN ERYTHROCYTES; MESSENGER-RNA; PLACENTA; INHIBITORS; EXPRESSION; GLUCOSE; SURFACE; LYSINE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
28
Recensione:
Indirizzi per estratti:
Indirizzo: Eaton, BM Chelsea & Westminster Hosp, Imperial Coll, Sch Med, Dept Maternal & Fetal Med, 369 Fulham Rd, London SW10 9NH, England Chelsea & Westminster Hosp 369 Fulham Rd London England SW10 9NH
Citazione:
B.M. Eaton e S.R. Sooranna, "Transport of large neutral amino acids into BeWo cells", PLACENTA, 21(5-6), 2000, pp. 558-564

Abstract

BeWo choriocarcinoma cells were cultured onto solid microcarrier beads, packed into columns and superfused. Unidirectional influx of L-phenylalanine (L-phe) and L-leucine (L-leu) across the microvillous border of the cells was studied using a rapid paired-tracer dilution technique. Influx of L-phe and L-leu comprised both saturable and non-saturable components. K-m valuesfor L-phe and L-leu were 0.57 +/- 0.01 mM and 0.05 +/- 0.01 mM, respectively, with V-max values of 120.4 +/- 0.5 nmol/mg/min and 41.7 +/- 0.2 nmol/mg/min. Non-saturable uptake components were 29.0 +/- 0.1 nmol/mg/mM and 37.9+/- 0.1 nmol/mg/min/mM respectively. L-leu uptake was found to be sodium-independent. The uptake of L-[H-3]phe was strongly inhibited (90-100 per cent) by unlabelled L-phe, D-phe, L-leu or 2-aminoendobicyclo-[2,2,1]-heptane-2-carboxylic acid (BCH) but not by L-arginine (L-arg) or methyl alpha-aminoisobutric acid (Me-AIB). Pre-incubation of Bewo cultures for 24 h in the presence of an additional 1.2 mtr L-phe (simulating maternal phenylketonuria)significantly reduced both the K-m and V-max components of L-phe influx. L-arg (2 mM) had no effect on L-leu influx whereas 2 mM L-phe completely inhibited saturable L-leu influx. These data suggest that the microvillous border of differentiated BeWo cells transport large neutral amino acids predominantly via system L rather than by B(0) or y(+)L transporters. (C) 2000 Harcourt Publishers Ltd.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/01/21 alle ore 07:46:07