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Titolo:
Comparative assessment of MHC antigen expression in bladder and testis tumour biopsies and established urological tumour cell lines: The relevance ofcytokine and gene transfection for correction of defective MHC antigens
Autore:
Nouri, AME; Symes, MO;
Indirizzi:
Royal London Hosp, Dept Med Oncol, London E1, England Royal London Hosp London England E1 , Dept Med Oncol, London E1, England
Titolo Testata:
ONCOLOGY REPORTS
fascicolo: 5, volume: 7, anno: 2000,
pagine: 959 - 964
SICI:
1021-335X(200009/10)7:5<959:CAOMAE>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
CLASS-I; LYMPHOCYTES-T; HLA ANTIGENS; CANCER; CARCINOMA; INTERLEUKIN-2; PROGRESSION; RESTRICTION; MELANOMA; P53;
Keywords:
MHC; transfection; interferon; bladder tumour; testis tumour;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
33
Recensione:
Indirizzi per estratti:
Indirizzo: Nouri, AME Royal London Hosp, Dept Med Oncol, London E1, England Royal London Hosp London England E1 Oncol, London E1, England
Citazione:
A.M.E. Nouri e M.O. Symes, "Comparative assessment of MHC antigen expression in bladder and testis tumour biopsies and established urological tumour cell lines: The relevance ofcytokine and gene transfection for correction of defective MHC antigens", ONCOL REP, 7(5), 2000, pp. 959-964

Abstract

The aim of this study using radio-binding (RB), peroxidase-anti-peroxidase(PAP) and immunoprecipitation (IP) techniques was to investigate the pattern of major histocompatibility complex (MHC) antigen expression in urological malignancies and to compare the results with those seen in established urological human tumour cell lines. The results showed that using PAP technique, the percent bladder cases showing complete loss or cases with greater than 90% of tumour cells negative with W6/32 (detects all class I antigens), HC10 (detects free heavy chain) and BMM.1 (detects beta 2-mirogobulin) monoclonal antibodies (Mab) were 16%, 44% and 2% respectively. In a subgroup of 37 cases, the intensity of MHC class II antigen expression for strong, weak and negative cases were 9 (24%), 8 (22%) and 20 (54%) respectively. Theexpression for class I antigens on testis tumours was mainly negative and when positive, it was present in a small percent of tumour cells. This was also observed for class II antigens where only 8% of cases showed some degree of positivity. Using RE technique, 10 of 12 (85%) of tumour lines expressed class I antigens spontaneously and following interferon gamma (IFN gamma) stimulation, the 2 negative lines one testis (Tera I) and one bladder (Fen) remained negative and 2 lines (both testis lines Tera II and Ep2102) showed a significant class I up-regulation. None of the lines expressed classII antigens spontaneously and following IFN gamma stimulation, 8 of 12 (66%) were induced. The absence of class I and II antigens in the negative lines was confirmed using IP technique. In the case of one class I negative bladder cell line i.e. Fen, the biochemical analysis showed the absence of beta 2-m gene product which could not be restored by IFN gamma stimulation. However, transfection of the cells with beta 2-m gene resulted in the expression of fully assembled class I antigens, indicating that the loss of antigens was due to the absence of functional beta 2-m gene. These results indicated the similarity between the pattern of expression of MHC antigens on tumour biopsies and established tumour cell lines. They also demonstrated that both cytokine stimulation and gene transfection could be used to correct defective class I antigens in tumour cell lines. These approaches might have important implications for preselection of bladder cancer patients for cytokine or gene therapies.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 08:59:07