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Titolo:
The extracellular domain of CD4 regulates the initiation of T cell activation
Autore:
Gratton, S; Haughn, L; Sekaly, RP; Julius, M;
Indirizzi:
Inst Rech Clin Montreal, Immunol Lab, Montreal, PQ H2W 1R7, Canada Inst Rech Clin Montreal Montreal PQ Canada H2W 1R7 al, PQ H2W 1R7, Canada McGill Univ, Dept Med, Div Expt Med, Montreal, PQ H3A 2T5, Canada McGill Univ Montreal PQ Canada H3A 2T5 Med, Montreal, PQ H3A 2T5, Canada Univ Toronto, Dept Immunol, Toronto, ON, Canada Univ Toronto Toronto ON Canada oronto, Dept Immunol, Toronto, ON, Canada Univ Hlth Network, Arthrit & Immune Disorder Res Ctr, Toronto, ON, Canada Univ Hlth Network Toronto ON Canada isorder Res Ctr, Toronto, ON, Canada
Titolo Testata:
MOLECULAR IMMUNOLOGY
fascicolo: 5, volume: 37, anno: 2000,
pagine: 213 - 219
SICI:
0161-5890(200004)37:5<213:TEDOCR>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
TYROSINE-PROTEIN-KINASE; AMINO-TERMINAL DOMAIN; HUMAN LYMPHOCYTES-T; SIGNAL TRANSDUCTION; ANTIGEN RECEPTOR; CYTOPLASMIC DOMAINS; CROSS-LINKING; ASSOCIATION; P56LCK; PHOSPHORYLATION;
Keywords:
CD4; TCR; Lck; activation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
40
Recensione:
Indirizzi per estratti:
Indirizzo: Sekaly, RP Inst Rech Clin Montreal, Immunol Lab, Montreal, PQ H2W 1R7, Canada Inst Rech Clin Montreal Montreal PQ Canada H2W 1R7 1R7, Canada
Citazione:
S. Gratton et al., "The extracellular domain of CD4 regulates the initiation of T cell activation", MOL IMMUNOL, 37(5), 2000, pp. 213-219

Abstract

To assess the respective contribution of the extracellular and intracellular domains of CD4 in regulating early T cell activation events, we have used a CD4-independent murine T cell clone transfected with human CD4. Stimulation of CD4 positive clones could only be observed if CD4 molecules associated to lck were co-aggregated with the TCR complex, confirming that the simultaneous interaction of MI-IC class II molecules with the CD4/lck complex and the TCR is required to initiate T cell activation. To assess the involvement of the extracellular portion of CD4 in this process, we transfected achimeric molecule (EGFRCD4) consisting of the extracellular portion of theepidermal growth factor receptor (EGFR), and of the transmembrane and cytoplasmic domains of human CD4. Although this chimeric molecule associates with lck, transfected clones were induced to proliferate by mAb specific for TCR in the absence of co-aggregation. A new regulatory role for the extracellular domain of CD4 which is independent of its interaction with MHC classII molecules is thus revealed in these experiments. Taken together, our results demonstrate that, in a CD4-independent cell line, two domains of CD4 regulate early T cell activation events: (1) its association with lck and (2) its extracellular domain, independently of its interaction with MHC class II molecules. (C) 2000 Published by Elsevier Science Ltd.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/04/20 alle ore 22:25:59