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Titolo:
Enantioselective analysis of disopyramide and mono-N-dealkyldisopyramide in plasma and urine by high-performance liquid chromatography on an amylose-derived chiral stationary phase
Autore:
Bortocan, R; Lanchote, VL; Cesarino, EJ; Bonato, PS;
Indirizzi:
USP, Fac Ciencias Farmaceut Ribeirao Preto, BR-14040903 Ribeirao Preto, Brazil USP Ribeirao Preto Brazil BR-14040903 BC-14040903 Ribeirao Preto, Brazil USP, Fac Med Ribeirao Preto, BR-14040903 Ribeirao Preto, Brazil USP Ribeirao Preto Brazil BR-14040903 BC-14040903 Ribeirao Preto, Brazil
Titolo Testata:
JOURNAL OF CHROMATOGRAPHY B
fascicolo: 2, volume: 744, anno: 2000,
pagine: 299 - 306
SICI:
1387-2273(20000721)744:2<299:EAODAM>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
ALPHA-1-ACID GLYCOPROTEIN COLUMN; ENANTIOMERS; PROTEIN; (R)-DISOPYRAMIDE; (S)-DISOPYRAMIDE; METABOLITE; BINDING; HUMANS;
Keywords:
chiral stationary phases; LC; enantiomer separation; disopyramide; mono-N-dealkyldisopyramide;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
18
Recensione:
Indirizzi per estratti:
Indirizzo: Bonato, PS USP, Fac Ciencias Farmaceut Ribeirao Preto, BR-14040903 Ribeirao Preto, Brazil USP Ribeirao Preto Brazil BR-14040903 BCibeirao Preto, Brazil
Citazione:
R. Bortocan et al., "Enantioselective analysis of disopyramide and mono-N-dealkyldisopyramide in plasma and urine by high-performance liquid chromatography on an amylose-derived chiral stationary phase", J CHROMAT B, 744(2), 2000, pp. 299-306

Abstract

An enantioselective high-performance liquid chromatography method was developed for the simultaneous determination of disopyramide (DP) and mono-N-dealkyldisopyramide (MND) enantiomers in plasma and urine. The drugs were extracted from plasma samples by liquid-liquid extraction with dichloromethaneafter protein precipitation with trichloroacetic acid; the urine samples were processed by Liquid-liquid extraction with dichloromethane. The enantiomers were resolved on a Chiralpak AD column using hexane-ethanol (91:9, v/v) plus 0.1% diethylamine as the mobile phase and monitored at 270 nm. Underthese conditions the enantiomeric fractions of the drug and of its metabolite were analyzed within 20 min. The extraction procedure was efficient in removing endogenous interferents and low values for the relative standard deviations were demonstrated for both within-day and between-day assays. Themethod described in this paper allows the determination of DP and MND enantiomers at plasma levels as low as 12.5 ng/ml and can be used in clinical pharmacokinetic studies. (C) 2000 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/04/20 alle ore 22:32:32