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Titolo:
Determination of twelve antiretroviral agents in human plasma sample usingreversed-phase high-performance liquid chromatography
Autore:
Aymard, G; Legrand, M; Trichereau, N; Diquet, B;
Indirizzi:
CHU Pitie Salpetriere, Assistance Publ Hop Paris, Serv Pharmacol Clin, LabPharmacocinet, F-75634 Paris 13, France CHU Pitie Salpetriere Paris France 13 acocinet, F-75634 Paris 13, France
Titolo Testata:
JOURNAL OF CHROMATOGRAPHY B
fascicolo: 2, volume: 744, anno: 2000,
pagine: 227 - 240
SICI:
1387-2273(20000721)744:2<227:DOTAAI>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
IMMUNODEFICIENCY-VIRUS PROTEASE; ULTRAVIOLET DETECTION; CEREBROSPINAL-FLUID; SIMULTANEOUS QUANTIFICATION; QUANTITATIVE-DETERMINATION; RAPID QUANTIFICATION; BIOLOGICAL SAMPLES; HIV-INFECTION; HUMAN SERUM; INDINAVIR;
Keywords:
protease inhibitors; nucleoside reverse transcriptase inhibitors; non-nucleoside reverse transcriptase inhibitors;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
50
Recensione:
Indirizzi per estratti:
Indirizzo: Diquet, B CHU Pitie Salpetriere, Assistance Publ Hop Paris, Serv PharmacolClin, LabPharmacocinet, 47-83 Bd Hop, F-75634 Paris 13, France CHU Pitie Salpetriere 47-83 Bd Hop Paris France 13 s 13, France
Citazione:
G. Aymard et al., "Determination of twelve antiretroviral agents in human plasma sample usingreversed-phase high-performance liquid chromatography", J CHROMAT B, 744(2), 2000, pp. 227-240

Abstract

A new high-performance liquid chromatography (HPLC) with UV detection assay was developed for the simultaneous determination of protease inhibitors (PIs), nucleoside and non-nucleoside reverse transcriptase inhibitors (NRTIs, NNRTIs) using a single 1-ml plasma samples. A solid-liquid extraction procedure without internal standard was coupled with two separate reversed-phase HPLC systems; one for the determination of amprenavir, efavirenz, indinavir, nelfinavir, ritonavir, saquinavir (run time=32 min) and one for the determination of abacavir, didanosine, lamivudine, stavudine, nevirapine, zidovudine (run time=40 min). The first requires a mobile phase containing sodium phosphate buffer+ion pair-acetonitrile (50:50, v/v) through a C-18 Symmetry column (250x4.6 mm I.D., 5 mu m particle size), using variable wavelengths (241, 254 and 261 nm). The second system requires three mobile phases (potassium phosphate buffer+ion pair-acetonitrile) for different elution through a C-18 Symmetry Shield column (250x4.6 mm I.D., 5 mu m), using a single wavelength (260 nm). Peak-areas are linear; correlation coefficients arebetter than 0.998 for all compounds, with both inter- and intra-day relative standard deviations lower than 12%. Extraction recoveries are higher than 93% for PIs and NNRTIs and higher than 70% for NRTIs. The method is specific and sensitive and was used to determine trough and peak levels of antiretroviral drugs in HIV infected patients under various combinations of RTIsand PIs. (C) 2000 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/01/20 alle ore 06:40:03