Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Molecular cloning of a gene encoding the thermoactive levansucrase from Rahnella aquatilis and its growth phase-dependent expression in Escherichia coli
Autore:
Seo, JW; Song, KB; Jang, KH; Kim, CH; Jung, BH; Rhee, SK;
Indirizzi:
Korea Res Inst Biosci & Biotechnol, Microbial & Bioproc Engn Lab, Taejon 305600, South Korea Korea Res Inst Biosci & Biotechnol Taejon South Korea 305600 South Korea
Titolo Testata:
JOURNAL OF BIOTECHNOLOGY
fascicolo: 1, volume: 81, anno: 2000,
pagine: 63 - 72
SICI:
0168-1656(20000728)81:1<63:MCOAGE>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
BACILLUS-SUBTILIS LEVANSUCRASE; ACETOBACTER-DIAZOTROPHICUS SRT4; PATHOGEN ERWINIA-AMYLOVORA; ZYMOMONAS-MOBILIS; NUCLEOTIDE-SEQUENCE; LEVAN; SECRETION; BACTERIA;
Keywords:
Rahnella aquatilis; levan; levansucrase gene (lsrA); growth phase-dependent expression;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
30
Recensione:
Indirizzi per estratti:
Indirizzo: Rhee, SK Korea Res Inst Biosci & Biotechnol, Microbial & Bioproc Engn Lab,POB 115,Taejon 305600, South Korea Korea Res Inst Biosci & Biotechnol POB 115 Taejon South Korea 305600
Citazione:
J.W. Seo et al., "Molecular cloning of a gene encoding the thermoactive levansucrase from Rahnella aquatilis and its growth phase-dependent expression in Escherichia coli", J BIOTECH, 81(1), 2000, pp. 63-72

Abstract

A levansucrase gene (lsrA) from Rahnella aquatilis ATCC33071 was isolated from a genomic library and the nucleotide sequence of the Isr A structural gene was determined. Isl A is composed of 1248 bp and encodes 415 amino acid residues with a calculated molecular mass of 45.9 kDa. Although the aminoacid sequence of lsrA gene showed good conservation with the sequences of reported levansucrases and of the conserved regions thought to be implicated in the enzyme activity, comparison of the deduced amino acid sequences certified the dissimilarity of the proteins from Gram-negative and Gram-positive bacteria. The Is,A gene was expressed from its own promoter in Escherichia coli in an active form. The lsrA expression in E. coli-pRL1CPR was affected by the growth phase of cells: it was repressed in the early phase of growth, but was significantly stimulated during the entrance of cells into the late phase of growth. The growth-phase-dependent fashion of Isi A expression was altered in a constitutive-like fashion by the deletion of an upstream region of lsrA (pNd137), suggesting that the growth-phase dependent expression of lsrA was mediated by the deleted upstream region. (C) 2000 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/04/20 alle ore 23:06:56