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Titolo:
Phosphorylation of collapsin response mediator protein-2 by Rho-kinase - Evidence for two separate signaling pathways for growth cone collapse
Autore:
Arimura, N; Inagaki, N; Chihara, K; Menager, C; Nakamura, N; Amano, M; Iwamatsu, A; Goshima, Y; Kaibuchi, K;
Indirizzi:
Nara Inst Sci & Technol, Div Signal Transduct, Ikoma 6300101, Japan Nara Inst Sci & Technol Ikoma Japan 6300101 nsduct, Ikoma 6300101, Japan Nagoya Univ, Sch Med, Dept Cell Pharmacol, Showa Ku, Nagoya, Aichi 4668550, Japan Nagoya Univ Nagoya Aichi Japan 4668550 a Ku, Nagoya, Aichi 4668550, Japan Yokohama City Univ, Sch Med, Dept Pharmacol, Kanazawa Ku, Yokohama, Kanagawa 2360004, Japan Yokohama City Univ Yokohama Kanagawa Japan 2360004 anagawa 2360004, Japan Kirin Brewery Co Ltd, Cent Labs Key Technol, Kanazawa Ku, Yokohama, Kanagawa 2360004, Japan Kirin Brewery Co Ltd Yokohama Kanagawa Japan 2360004 agawa 2360004, Japan
Titolo Testata:
JOURNAL OF BIOLOGICAL CHEMISTRY
fascicolo: 31, volume: 275, anno: 2000,
pagine: 23973 - 23980
SICI:
0021-9258(20000804)275:31<23973:POCRMP>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
ACTIN STRESS FIBERS; INDUCED NEURITE RETRACTION; MYOTONIC-DYSTROPHY KINASE; FAMILY GTPASES; AXON GUIDANCE; CYTOSKELETAL REORGANIZATION; SERINE/THREONINE KINASE; LYSOPHOSPHATIDIC ACID; FOCAL ADHESIONS; PUTATIVE TARGET;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
53
Recensione:
Indirizzi per estratti:
Indirizzo: Kaibuchi, K Nara Inst Sci & Technol, Div Signal Transduct, 8916-5 Takayama, Ikoma 6300101, Japan Nara Inst Sci & Technol 8916-5 Takayama Ikoma Japan6300101 n
Citazione:
N. Arimura et al., "Phosphorylation of collapsin response mediator protein-2 by Rho-kinase - Evidence for two separate signaling pathways for growth cone collapse", J BIOL CHEM, 275(31), 2000, pp. 23973-23980

Abstract

We previously identified Rho-associated protein kinase (Rho-kinase) as a specific effector of Rho, In this study, we identified collapsin response mediator protein-2 (CRMP-2), as a novel Rho-kinase substrate in the brain. CRMP-2 is a neuronal protein whose expression is up-regulated during development. Rho-kinase phosphorylated CRMP-2 at Thr-555 in vitro. We produced an antibody that specifically recognizes CRMP-2 phosphorylated at Thr-555, Using this antibody, we found that Rho-kinase phosphorylated CRMP-2 downstream of Rho in COS7 cells. Phosphorylation of CRMP-2 was observed in chick dorsal root ganglion neurons during lysophosphatidic acid (LPA)-induced growth cone collapse, whereas the phosphorylation was not detected during semaphorin-3A-induced growth cone collapse. Both LPA-induced CRMP-2 phosphorylation and LPA-induced growth cone collapse were inhibited by Rho-kinase inhibitorUA1077 or Y-32885. LPA-induced growth cone collapse was also blocked by a dominant negative form of Rho-kinase. On the other hand, semaphorin-3A-induced growth cone collapse was not inhibited by a dominant negative form of Rho-kinase, Furthermore, overexpression of a mutant CRMP-2 in which Thr-555 was replaced by Ala significantly inhibited LPA-induced growth cone collapse. These results demonstrate the existence of Rho-kinase-dependent and -independent pathways for growth cone collapse and suggest that CRMP-2 phosphorylation by Rho-kinase is involved in the former pathway.

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Documento generato il 20/01/21 alle ore 10:33:43