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Titolo:
A novel electrogenic amino acid transporter is activated by K+ or Na+, is alkaline pH-dependent, and is Cl--independent
Autore:
Feldman, DH; Harvey, WR; Stevens, BR;
Indirizzi:
Univ Florida, Coll Med, Dept Physiol, Gainesville, FL 32652 USA Univ Florida Gainesville FL USA 32652 Physiol, Gainesville, FL 32652 USA Univ Florida, Whitney Lab, St Augustine, FL 32086 USA Univ Florida St Augustine FL USA 32086 ey Lab, St Augustine, FL 32086 USA
Titolo Testata:
JOURNAL OF BIOLOGICAL CHEMISTRY
fascicolo: 32, volume: 275, anno: 2000,
pagine: 24518 - 24526
SICI:
0021-9258(20000811)275:32<24518:ANEAAT>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
PLASMA-MEMBRANE ENERGIZATION; L-PROLINE TRANSPORTER; GATED ION-CHANNEL; SEROTONIN TRANSPORTERS; XENOPUS-OOCYTES; GABA TRANSPORTER; V-ATPASES; CLONING; EXPRESSION; COTRANSPORTER;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
48
Recensione:
Indirizzi per estratti:
Indirizzo: Stevens, BR Univ Florida, Coll Med, Dept Physiol, 1600 SW Archer Rd,Box 100274, Gainesville, FL 32652 USA Univ Florida 1600 SW Archer Rd,Box 100274 Gainesville FL USA 32652
Citazione:
D.H. Feldman et al., "A novel electrogenic amino acid transporter is activated by K+ or Na+, is alkaline pH-dependent, and is Cl--independent", J BIOL CHEM, 275(32), 2000, pp. 24518-24526

Abstract

A new eukaryotic nutrient amino acid transporter has been cloned from an epithelium that is exposed to high voltages and alkaline pH. The full-lengthcDNA encoding this navel CAATCH1 ((c) over bar ation-(a) over bar nion-activated (A) over bar mino acid (t) over bar ransporter/<(ch)over bar>annel) was isolated using a polymerase chain reaction-based strategy, and its expression product in Xenopus oocytes displayed a combination of several unique, unanticipated functional properties. CAATCH1 electrophysiological properties resembled those of Na+,Cl--coupled neurotransmitter amine transporters,although CAATCH1 was cloned from a gut absorptive epithelium rather than from an excitable tissue. Amino acids such as L-proline, L-threonine, and L-methionine elicited complex current-voltage relationships in alkaline pH-dependent CAATCH1 that were reminiscent of the behavior of the dopamine, serotonin, and norepinephrine transporters (DAT, SERT, NET) in the presence of their substrates and pharmacological inhibitors such as cocaine or antidepressants, These I-V relationships indicated a combination of substrate-associated carrier current plus an independent CAATCH1-associated leakage current that could be blocked by certain amino acids. However, unlike all structurally related proteins, CAATCH1 activity is absolutely independent of Cl-. Unlike related KAAT1, CAATCH1 possesses a methionine-inhibitable constitutive leakage current and is able to switch its narrow substrate selectivity, preferring threonine in the presence of K+ but preferring proline in the presence of Na+.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/04/20 alle ore 04:37:39