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Titolo:
Mechanism of kappa B DNA binding by Rel/NF-kappa B dimers
Autore:
Phelps, CB; Sengchanthalangsy, LL; Malek, S; Ghosh, G;
Indirizzi:
Univ Calif San Diego, Dept Chem & Biochem, La Jolla, CA 92093 USA Univ Calif San Diego La Jolla CA USA 92093 iochem, La Jolla, CA 92093 USA
Titolo Testata:
JOURNAL OF BIOLOGICAL CHEMISTRY
fascicolo: 32, volume: 275, anno: 2000,
pagine: 24392 - 24399
SICI:
0021-9258(20000811)275:32<24392:MOKBDB>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
TRANSCRIPTION FACTOR; P50 HOMODIMER; P50/P65 HETERODIMER; ACTIVATION; DIMERIZATION; PROTEINS; COMPLEX; SUBUNIT; SITE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
25
Recensione:
Indirizzi per estratti:
Indirizzo: Ghosh, G Univ Calif San Diego, Dept Chem & Biochem, La Jolla, CA 92093 USAUniv Calif San Diego La Jolla CA USA 92093 a Jolla, CA 92093 USA
Citazione:
C.B. Phelps et al., "Mechanism of kappa B DNA binding by Rel/NF-kappa B dimers", J BIOL CHEM, 275(32), 2000, pp. 24392-24399

Abstract

The DNA binding of three different NF-KB dimers, the p50 and p65 homodimers and the p50/p65 heterodimer, has been examined using a combination of gelmobility shift and fluorescence anisotropy assays. The NF-KB p50/p65 heterodimer is shown here to bind the KB DNA target site of the immunoglobulin kappa enhancer (Ig-KB) with an affinity of approximately 10 nM. The p50 and p65 homodimers bind to the same site with roughly 5- and 15-fold lower affinity, respectively. The nature of the binding isotherms indicates a cooperative mode of binding for all three dimers to the DNA targets. We have further characterized the role of pH, salt, and temperature on the formation of the p50/p65 heterodimer-Ig-KB complex. The heterodimer binds to the Ig-KB DNA target in a pH-dependent manner, with the highest affinity between pH 7.0 and 7.5. A strong salt-dependent interaction between Ig-KB and the p50/p65 heterodimer is observed, with optimum binding occurring at monovalent salt concentrations below 75 mM, with binding becoming virtually nonspecific at a salt concentration of 200 mM. Binding of the heterodimer to DNA was unchanged across a temperature range between 4 degrees C and 42 degrees C. Thesensitivity to ionic environment and insensitivity to temperature indicatethat NF-KB p50/p65 heterodimers form complexes with specific DNA in an entropically driven manner.

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Documento generato il 04/07/20 alle ore 23:33:56