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Titolo:
The membrane-bound H+-ATPase complex is essential for growth of Lactococcus lactis
Autore:
Koebmann, BJ; Nilsson, D; Kuipers, OP; Jensen, PR;
Indirizzi:
Tech Univ Denmark, Dept Microbiol, DK-2800 Lyngby, Denmark Tech Univ Denmark Lyngby Denmark DK-2800 robiol, DK-2800 Lyngby, Denmark Chr Hansen AS, Dept Physiol, DK-2970 Horsholm, Denmark Chr Hansen AS Horsholm Denmark DK-2970 hysiol, DK-2970 Horsholm, Denmark NIZO Food Res, Microbial Ingredients Sect, NL-6710 BA Ede, Netherlands NIZO Food Res Ede Netherlands NL-6710 BA ct, NL-6710 BA Ede, Netherlands
Titolo Testata:
JOURNAL OF BACTERIOLOGY
fascicolo: 17, volume: 182, anno: 2000,
pagine: 4738 - 4743
SICI:
0021-9193(200009)182:17<4738:TMHCIE>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROTON-TRANSLOCATING ATPASE; ESCHERICHIA-COLI; STREPTOCOCCUS-FAECALIS; CYTOPLASMIC PH; NUCLEOTIDE-SEQUENCE; GENE-EXPRESSION; ALPHA-SUBUNITS; SYNTHASE; OPERON; ORGANIZATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
43
Recensione:
Indirizzi per estratti:
Indirizzo: Jensen, PR Tech Univ Denmark, Dept Microbiol, Bldg 301, DK-2800 Lyngby, Denmark Tech Univ Denmark Bldg 301 Lyngby Denmark DK-2800 gby, Denmark
Citazione:
B.J. Koebmann et al., "The membrane-bound H+-ATPase complex is essential for growth of Lactococcus lactis", J BACT, 182(17), 2000, pp. 4738-4743

Abstract

The eight genes which encode the (F1F0) H+-ATPase in Lactococcus lactis subsp, cremoris MG1363 were cloned and sequenced. The genes were organized inan operon with the gene order atpEBFNAGDC; i.e., the order of atpE and atpB is reversed with respect to the more typical bacterial organization. The deduced amino acid sequences of the corresponding H+-ATPase subunits showedsignificant homology with the subunits from other organisms. Results of Northern blot analysis showed a transcript at approximately 7 kb, which corresponds to the size of the atp operon, The transcription initiation site wasmapped by primer extension and coincided with a standard promoter sequence. In order to analyze the importance of the H+-ATPase for L, lactis physiology, a mutant strain was constructed in which the original atp promoter on the chromosome was replaced with an inducible nisin promoter. When grown onGM17 plates the resulting strain was completely dependent on the presence of nisin for growth. These data demonstrate that the H+-ATPase is essentialfor growth oft. lactis under these conditions.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/06/20 alle ore 01:29:30