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Titolo:
Celery allergens in patients with positive double-blind placebo-controlledfood challenge
Autore:
Luttkopf, D; Ballmer-Weber, BK; Wuthrich, B; Vieths, S;
Indirizzi:
Paul Ehrlich Inst, Dept Allergol, D-63225 Langen, Germany Paul Ehrlich Inst Langen Germany D-63225 lergol, D-63225 Langen, Germany Univ Zurich Hosp, Dept Allergol, Allergy Unit, CH-8091 Zurich, SwitzerlandUniv Zurich Hosp Zurich Switzerland CH-8091 CH-8091 Zurich, Switzerland
Titolo Testata:
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY
fascicolo: 2, volume: 106, anno: 2000,
pagine: 390 - 399
SICI:
0091-6749(200008)106:2<390:CAIPWP>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
REACTIVE CARBOHYDRATE DETERMINANTS; BIRCH POLLEN ALLERGEN; CROSS-REACTIVITY; MAJOR ALLERGEN; CLINICAL RELEVANCE; VEGETABLE FOODS; TREE POLLEN; N-GLYCANS; IGE; IDENTIFICATION;
Keywords:
celery allergy; double-blind placebo-controlled food challenge; Api g 1; Api g 4; carbohydrate epitopes; cross-reactive carbohydrate determinants; IgE; enzyme allergosorbent test; immunoblotting;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
47
Recensione:
Indirizzi per estratti:
Indirizzo: Vieths, S Paul Ehrlich Inst, Dept Allergol, Paul Ehrlich Str 51, D-63225 Langen, Germany Paul Ehrlich Inst Paul Ehrlich Str 51 Langen Germany D-63225 ny
Citazione:
D. Luttkopf et al., "Celery allergens in patients with positive double-blind placebo-controlledfood challenge", J ALLERG CL, 106(2), 2000, pp. 390-399

Abstract

Background: Recently, for the first time, allergy to celery was confirmed by double-blind placebo-controlled food challenge (DBPCFC), Api g 1, Api g 4, cross-reactive carbohydrate determinants (CCD), and a 60 kDa allergen have been described as celery allergens. Objective: To get insights in IgE responses of patients with a positive DBPCFC to celery tuber (celeriac) compared with patients with a negative challenge test,Methods: Specific IgE to native and heated celery tuber and to recombinantApi g 1, the major celery allergen, were determined by enzyme allergosorbent test and immunoblotting, IgE binding to Api g 1, Api g 4, and CCD was confirmed by inhibition experiments that used recombinant Api g 1, recombinant Api g 4, pure N-glycans, and extracts of celeriac, lychee fruit, and pollens of birch, mugwort, and timothy grass as inhibitors. Results: Immunoblotting with sera from 22 patients with a positive DBPCFC to celeriac confirmed the presence of known allergenic structures: The major allergen Api g 1 (16 kDa) was recognized by IgE from 13 of 22 patients (59%), Another major allergen was CCD, determined by IgE reactivity in 12 of 22 patients (55%), Celery profilin, Api g 4, was recognized by IgE from 5 of 22 patients (23%),Conclusion: Our DBPCFC-positive patients exclusively presented IgE to known celery allergens, although the prevalences were slightly different than were previously reported. No obvious differences were found in patients withpositive IgE antibody but negative challenge test, IgE binding to all 3 structures in celeriac extract was inhibited by birch pollen extract, whereasmugwort pollen extract could only inhibit IgE reactivity to Api g 4 and CCD, Inhibition experiments with a purified carbohydrate moiety clearly showed that the IgE epitope mannose-xylose-fucose-glycan (Man alpha 1-6[Xyl beta1-2]Man beta 1-4GlcNAc beta 1-4[Fuc alpha 1-3]GlcNAc) or a closely relatedstructure is present in celeriac extract and is important in patients withclinical allergy to celery.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/01/21 alle ore 00:09:20