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Titolo:
Spontaneous apoptosis of Reed-Sternberg and Hodgkin cells; clinical and pathological implications in patients with Hodgkin's disease
Autore:
Smolewski, P; Niewiadomska, H; Los, E; Robak, T;
Indirizzi:
Med Univ Lodz, Dept Hematol, Lodz, Poland Med Univ Lodz Lodz PolandMed Univ Lodz, Dept Hematol, Lodz, Poland Med Univ Lodz, Dept Oncol, Lodz, Poland Med Univ Lodz Lodz PolandMed Univ Lodz, Dept Oncol, Lodz, Poland Med Univ Lodz, Inst Pediat, Pathol Lab, Lodz, Poland Med Univ Lodz Lodz Poland v Lodz, Inst Pediat, Pathol Lab, Lodz, Poland
Titolo Testata:
INTERNATIONAL JOURNAL OF ONCOLOGY
fascicolo: 3, volume: 17, anno: 2000,
pagine: 603 - 609
SICI:
1019-6439(200009)17:3<603:SAORAH>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
EPSTEIN-BARR-VIRUS; PROGNOSTIC-SIGNIFICANCE; PROTEIN EXPRESSION; MDM-2 PROTEINS; P53; LYMPHOMAS; P21; DEATH; BCL-2; P21(WAF1/CIP1);
Keywords:
Hodgkin's disease; TUNEL assay; apoptosis; prognostic factors;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
30
Recensione:
Indirizzi per estratti:
Indirizzo: Smolewski, P New York Med Coll, Brander Canc Res Inst, 19 Bradhurst Ave, Hawthorne, NY 10532 USA New York Med Coll 19 Bradhurst Ave Hawthorne NY USA 10532 USA
Citazione:
P. Smolewski et al., "Spontaneous apoptosis of Reed-Sternberg and Hodgkin cells; clinical and pathological implications in patients with Hodgkin's disease", INT J ONCOL, 17(3), 2000, pp. 603-609

Abstract

The experiments were designed to study correlation between frequency of apoptosis of Reed-Sternberg/Hodgkin (R-S/H) cells, EBV infection of these cells, expression of the key proteins involved in regulation of apoptosis and cell cycle in R-S/H cells, the patients' pretreatment markers and the clinical outcome. One hundred and ten Hodgkin's disease (HD) patients were studies, of which 69 obtained complete remission (CR) after first-line treatmentand 41 did not respond. The time of follow-up was from 18 to 242, median 69.7, months. Apoptosis was evaluated by TUNEL technique (TdT-mediated dUTP nick end labeling) and the presence of EBV-latent membrane protein 1 as well as expression of Bcl-2, tumor suppressor p53, p21(WAF1), MDM-2, Rb1, PCNA, p27(KIP1) and caspase-3, was detected immunocytochemically on paraffin-embedded lymph node specimens obtained at diagnosis. Positive TUNEL reaction was found in 43 patients with apoptotic index (AI) in this group varying between 10% and 60%. In the remaining 57 patients AI of R-S/H cells was below10%. In 62 patients the cells surrounding R-S/H cells were also TUNEL-positive; their frequency was variable. The expression of LMP1 protein on R-S/Hcells was found in 38 patients, without any correlation with the presence or frequency of apoptosis. No significant difference was seen between the AI and both clinical stage and histological type of the disease. However, the mean AI in non-responding patients was significantly higher than in CR group (p=0.015); the high frequency of apoptosis was also negatively correlated with the progression free survival time (p=0.031) and the overall survival (p=0.042). The expression of PCNA, p21(WAF1), p53 protein and caspase-3 also showed positive correlation with frequency of apoptosis (p=0.011, p=0.036, and p=0.001, respectively). On the other hand, no statistically confirmed correlation was found between AI and expression of bcl-2, MDM-2, Rb1, and p27(KIP1) On R-S/H cells. These data provide evidence that tumor cells in HD undergo spontaneous apoptosis regardless of EBV infection. High pretreatment AI correlates with poor response to the treatment, and may be considered as a potential negative prognostic factor in HD.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/11/20 alle ore 09:48:20