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Titolo:
Temporal and spatial regulation of expression of two galectins during kidney development of the chicken
Autore:
Stierstorfer, B; Kaltner, H; Neumuller, C; Sinowatz, F; Gabius, HJ;
Indirizzi:
Univ Munich, Tierarztliche Fak, Inst Tieranat 2, D-80539 Munich, Germany Univ Munich Munich Germany D-80539 t Tieranat 2, D-80539 Munich, Germany Univ Munich, Tierarztliche Fak, Inst Physiol Chem, D-80539 Munich, GermanyUniv Munich Munich Germany D-80539 Physiol Chem, D-80539 Munich, Germany
Titolo Testata:
HISTOCHEMICAL JOURNAL
fascicolo: 6, volume: 32, anno: 2000,
pagine: 325 - 336
SICI:
0018-2214(200006)32:6<325:TASROE>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
GALACTOSIDE-BINDING LECTIN; HEPARIN ENDOGENOUS LECTINS; ANIMAL LECTINS; EMBRYONIC-DEVELOPMENT; IMMUNOHISTOCHEMICAL LOCALIZATION; BIOTINYLATED NEOGLYCOPROTEINS; CEREBELLAR ONTOGENY; HUMAN-PLACENTA; CELL; PROTEIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
89
Recensione:
Indirizzi per estratti:
Indirizzo: Sinowatz, F Univ Munich, Tierarztliche Fak, Inst Tieranat 2, Vet Str 13, D-80539 Munich, Germany Univ Munich Vet Str 13 Munich Germany D-80539 Munich, Germany
Citazione:
B. Stierstorfer et al., "Temporal and spatial regulation of expression of two galectins during kidney development of the chicken", HISTOCHEM J, 32(6), 2000, pp. 325-336

Abstract

Organogenesis and the establishment of the mature phenotype require an interplay between diverse recognition systems. Concerning protein-carbohydrateinteractions, galectins are known to be involved in several extra- and intracellular functions. Due to the occurrence of two avian galectins in liver(chicken galectin-16; CG-16) and intestine (chicken galectin- 14; CG-14) with different developmental regulation, the questions addressed are to whatextent and where these galectins are present during chicken kidney development. Using Western blot analysis, the presence of both activities in tissue extracts was ascertained. A solid-phase assay showed peak levels at day 12 followed by a decline. A histochemical analysis was carried out in combination with routine staining. Epithelial cells of the mesonephric proximal tubules were immunoreactive in the cytoplasm for CG-14 from day 5 of incubation onwards. Additionally, epithelial cells of the metanephric collecting ducts were stained. For CG-16 a rather similar pattern of staining was seen,additional positivity in early glomerular podocytes being notable. At the electron microscopical level, a diffuse staining for CG-14 was seen in the cytoplasm, whereas immunoreactivity for CG-16 was observed mainly in mitochondria. These results demonstrate quantitative differences in the developmental regulation of the two avian galectins with obvious similarities in thecell-type pattern but with a disparate intracellular localisation profile.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/12/20 alle ore 15:42:41