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Titolo:
A two dimensional electrophoresis database of a human Jurkat T-cell line
Autore:
Thiede, B; Siejak, F; Dimmler, C; Jungblut, PR; Rudel, T;
Indirizzi:
Max Planck Inst Infekt Biol, D-10117 Berlin, Germany Max Planck Inst Infekt Biol Berlin Germany D-10117 10117 Berlin, Germany
Titolo Testata:
ELECTROPHORESIS
fascicolo: 13, volume: 21, anno: 2000,
pagine: 2713 - 2720
SICI:
0173-0835(200007)21:13<2713:ATDEDO>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
RESOLUTION 2-DIMENSIONAL ELECTROPHORESIS; PROTEIN IDENTIFICATION; GEL-ELECTROPHORESIS; APOPTOSIS; PROTEOMICS; ACTIVATION;
Keywords:
two-dimensional electrophoresis database peptide mass fingerprinting; Jurkat T-cells; proteome; matrix assisted laser desorption/ionization-mass spectrometry;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
22
Recensione:
Indirizzi per estratti:
Indirizzo: Rudel, T Max Planck Inst Infekt Biol, Monbijoustr 2, D-10117 Berlin, Germany Max Planck Inst Infekt Biol Monbijoustr 2 Berlin Germany D-10117
Citazione:
B. Thiede et al., "A two dimensional electrophoresis database of a human Jurkat T-cell line", ELECTROPHOR, 21(13), 2000, pp. 2713-2720

Abstract

About 2000 protein spots of human Jurkat T-cells were detected by high resolution two-dimensional gel electrophoresis (2-DE) and were characterized in terms of their isoelectric point and molecular mass. A 2-DE database was constructed and is available at http://www.mpiib-berlin.mpg.de/2D-PAGE/. Atpresent the database contains 67 identified protein spots. These proteins were identified after tryptic digestion by peptide mass fingerprinting withdelayed extraction-matrix assisted laser desorption/ionization-mass spectrometry (DE-MALDI-MS). Proteins with a sequence coverage of at least 30% were introduced in the database. This sequence coverage could not always be obtained by using only the matrix alpha-cyano-4-hydroxycinnamic acid (CHCA) for the mass analysis. Therefore, an additional mass spectrum was recorded by using 2,5-dihydroxybenzoic acid (DHB). Usually, additional mass peaks were detected and together with the mass spectrum of CHCA this resulted in thedesired sequence coverage.

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Documento generato il 04/12/20 alle ore 22:21:46