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Titolo:
In vitro coupled transcription translation: Effects of modification in lysate preparation on protein composition and biosynthesis activity
Autore:
Schindler, PT; Baumann, S; Reuss, M; Siemann, M;
Indirizzi:
Univ Stuttgart, Inst Bioverfahrenstech, D-70569 Stuttgart, Germany Univ Stuttgart Stuttgart Germany D-70569 ech, D-70569 Stuttgart, Germany
Titolo Testata:
ELECTROPHORESIS
fascicolo: 13, volume: 21, anno: 2000,
pagine: 2606 - 2609
SICI:
0173-0835(200007)21:13<2606:IVCTTE>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Keywords:
cell-free protein synthesis; Escherichia coli; two-dimensional electrophoresis; green fluorescent proteins; elongation factor; protein phosphorylation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
9
Recensione:
Indirizzi per estratti:
Indirizzo: Siemann, M Univ Stuttgart, Inst Bioverfahrenstech, Allmandring 31, D-70569Stuttgart,Germany Univ Stuttgart Allmandring 31 Stuttgart Germany D-70569ermany
Citazione:
P.T. Schindler et al., "In vitro coupled transcription translation: Effects of modification in lysate preparation on protein composition and biosynthesis activity", ELECTROPHOR, 21(13), 2000, pp. 2606-2609

Abstract

Cell-free extracts (lysates) from Escherichia coil were used for protein synthesis in vitro. Essential steps of the lysate preparation were modified and analyzed with respect to their impact on in vitro protein synthesis capacity, using the green fluorescent protein (GFP) as a target protein. Variably manufactured lysates of low, medium and higher protein synthesis activity, were examined by high resolution two-dimensional get electrophoresis todetermine whether the modifications result in substantial alterations in protein composition of the final lysate. The total number of proteins calculated from the get maps did not vary for lysates with different activity andthus cannot serve as an evaluation parameter. Ribosomal proteins RP-S1, RP-L9, and RP-L10 were found in stoichiometric amounts for each of these lysates and in equal concentrations in comparison among the different lysates. Conversely, depending on the activity profiles, up to 7 different isoforms of the elongation factor EF-Ts were detected in the gel maps.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/11/20 alle ore 18:40:44