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Titolo:
Metaphase I arrest and spontaneous parthenogenetic activation of strain LTXBO oocytes: Chimeric reaggregated ovaries establish primary lesion in oocytes
Autore:
Eppig, JJ; Wigglesworth, K; Hirao, Y;
Indirizzi:
Jackson Lab, Bar Harbor, ME 04609 USA Jackson Lab Bar Harbor ME USA 04609Jackson Lab, Bar Harbor, ME 04609 USA
Titolo Testata:
DEVELOPMENTAL BIOLOGY
fascicolo: 1, volume: 224, anno: 2000,
pagine: 60 - 68
SICI:
0012-1606(20000801)224:1<60:MIAASP>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
GROWTH-DIFFERENTIATION FACTOR-9; GRANULOSA-CELL STEROIDOGENESIS; LT MOUSE OOCYTES; SECONDARY OOCYTES; MAMMALIAN OVARY; FOLLICLE CELLS; GAP JUNCTIONS; LT/SV MICE; CUMULUS; VITRO;
Keywords:
oocyte; meiosis; metaphase I arrest; parthenogenesis; chimeric reaggregated ovaries; strain LT; mouse;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
46
Recensione:
Indirizzi per estratti:
Indirizzo: Eppig, JJ Jackson Lab, 600 Main St, Bar Harbor, ME 04609 USA Jackson Lab 600 Main St Bar Harbor ME USA 04609 or, ME 04609 USA
Citazione:
J.J. Eppig et al., "Metaphase I arrest and spontaneous parthenogenetic activation of strain LTXBO oocytes: Chimeric reaggregated ovaries establish primary lesion in oocytes", DEVELOP BIO, 224(1), 2000, pp. 60-68

Abstract

Oocytes of strain LT mice, and related strains such as LTXBO, exhibit a high incidence of arrest in the progression of meiosis at metaphase I (MI) and in spontaneous parthenogenetic activation. Activation of these oocytes within the ovary leads to the formation of ovarian teratomas. In this study, the role of the oocyte's companion granulosa cells, the cumulus cells, was investigated using fully grown oocytes matured in vitro after isolation from LTXBO mice. Results showed that the role of cumulus cells in MI arrest isdichotomous. Cumulus cells temporarily helped to sustain MI arrest, but they also promoted a delayed progression to metaphase II. Cumulus cells also promoted parthenogenetic activation that occurred in association with the delayed progression to metaphase II. Next, the question of whether the lesion(s) promoting MI arrest and spontaneous activation is due to defects in the somatic cells or is intrinsic to the oocyte was addressed using chimeric reaggregated ovaries. An improved method for completely exchanging the germcell and the somatic cell compartments of ovaries from newborn mite is described. These chimeric reaggregated ovaries, grafted beneath the renal capsule of SCID mice, allowed the complete development of LTXBO oocytes to occur in association with somatic cells from control (B6SJLF(1)) ovaries and development of control oocytes in association with LTXBO somatic cells. Oocyte growth and follicular development appeared generally normal in reaggregated ovaries. High incidences of MI arrest and spontaneous activation of LTXBO oocytes occurred regardless of the genotype of the somatic cells. Moreover, there was a low incidence of MI arrest and spontaneous activation of control oocytes, even though they underwent complete development and maturation associated with LTXBO somatic cells. It is concluded that the phenotypes of MI arrest and parthenogenetic activation in LTXBO oocytes are defects caused by lesions intrinsic to the oocyte. Nevertheless, the oocyte's companion somatic cells play crucial roles in the expression of these lesions. (C)2000 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/11/20 alle ore 10:03:00