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Titolo:
A cDNA located on chromosome 7q32 shows loss of expression in epithelial cell line of nasopharyngeal carcinoma
Autore:
Jiang, N; Zhan, FH; Tan, GL; Deng, LW; Zhou, M; Cao, L; Qui, YZ; Xie, Y; Li, GY;
Indirizzi:
Hunan Med Univ, Canc Res Inst, Changsha 410078, Peoples R China Hunan Med Univ Changsha Peoples R China 410078 a 410078, Peoples R China Hunan Med Univ, Xiangya Hosp, Changsha 410078, Peoples R China Hunan Med Univ Changsha Peoples R China 410078 a 410078, Peoples R China
Titolo Testata:
CHINESE MEDICAL JOURNAL
fascicolo: 7, volume: 113, anno: 2000,
pagine: 650 - 653
SICI:
0366-6999(200007)113:7<650:ACLOC7>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
TUMOR-SUPPRESSOR GENES;
Keywords:
nasopharyngeal carcinoma; EST; clone; loss of heterozygosity; tumor supressor gene;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
15
Recensione:
Indirizzi per estratti:
Indirizzo: Jiang, N Hunan Med Univ, Canc Res Inst, Changsha 410078, Peoples R China Hunan Med Univ Changsha Peoples R China 410078 Peoples R China
Citazione:
N. Jiang et al., "A cDNA located on chromosome 7q32 shows loss of expression in epithelial cell line of nasopharyngeal carcinoma", CHIN MED J, 113(7), 2000, pp. 650-653

Abstract

Objective To isolate and clone the tumor suppressor gene on chromosomal region 7q32 correlated with the carcinogenesis of human nasopharyngeal carcinoma (NPC). Methods The genotypes of polymorphic microsatellite markers on 7q32 in DNAfrom 24 biopsies of nasopharyngeal carcinoma and matched normal blood cells were identified. The expression levels of 20 expressed sequence tags (ESTs) on 7q32 between human nasopharyngeal carcinoma epithelial 1 (HNE1) and primary cultures of normal nasopharyngeal epithelial ( PNNE) cells were compared using differential RT-PCR and Northern hybridization. The quantity of AA070437 DNA and mRNA was detected by differential PCR and differential RT-PCR, respectively. Results Loss of heterozygosity (LOH) was found in 25% - 46% of NPC biopsies. AA070437 EST expression was down-regulated in HNE1 cell compared to PNNEcells. The down-regulation of AA070437 was found in 30.7% of NPC biopsies and allelic loss of AA070437 was observed in 29.1% of NPC biopsies. Conclusion Our results show that AA070437 EST is negatively related with the occurrence of human NPC and may represent a candidate tumor suppressor gene of NPC on 7q32.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/04/20 alle ore 11:36:57