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Titolo:
Kinetics of BRCA1 regulation in response to UVC radiation
Autore:
Clarkin, CEK; Zhang, H; Weber, BL;
Indirizzi:
Univ Penn, Dept Med, Philadelphia, PA 19104 USA Univ Penn Philadelphia PAUSA 19104 Dept Med, Philadelphia, PA 19104 USA Univ Penn, Dept Biochem & Mol Biophys, Philadelphia, PA 19104 USA Univ Penn Philadelphia PA USA 19104 l Biophys, Philadelphia, PA 19104 USA Univ Penn, Dept Genet, Philadelphia, PA 19104 USA Univ Penn Philadelphia PA USA 19104 ept Genet, Philadelphia, PA 19104 USA
Titolo Testata:
CELLULAR AND MOLECULAR LIFE SCIENCES
fascicolo: 7, volume: 57, anno: 2000,
pagine: 1126 - 1134
SICI:
1420-682X(200007)57:7<1126:KOBRIR>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
DNA-DAMAGING AGENTS; CELL-CYCLE; CANCER CELLS; NUCLEAR PHOSPHOPROTEIN; FAMILIAL BREAST; OVARIAN-CANCER; MEIOTIC CELLS; EXPRESSION; REPAIR; P53;
Keywords:
breast cancer; BRCA1; DNA damage; ultraviolet C; MCF-7;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
36
Recensione:
Indirizzi per estratti:
Indirizzo: Weber, BL Univ Penn, Dept Med, Philadelphia, PA 19104 USA Univ Penn Philadelphia PA USA 19104 Philadelphia, PA 19104 USA
Citazione:
C.E.K. Clarkin et al., "Kinetics of BRCA1 regulation in response to UVC radiation", CELL MOL L, 57(7), 2000, pp. 1126-1134

Abstract

To investigate changes in BRCA1 following DNA damage, we exposed MCF-7 cells to increasing doses of ultraviolet C. We observed an increase in BRCA1 protein levels above 78 J/m(2). This increase was observed as early as 5 minafter irradiation. BRCA1 levels were then observed to decrease after 2 hi consistent with the previously published data. By pretreating with cycloheximide prior to irradiation, we observed a decrease in the protein half-life, from 3.5 h to 53 min, suggesting that a decrease in protein half-life maycause the lower levels of BRCA1 after irradiation. We also observed an increase in BRCA1 mRNA within 15 min of il radiation, followed by a decrease after 4 h. These data suggest that newly translated protein may contribute to increases in BRCA1 protein levels. The very rapid changes in BRCA1 support its role as a sensor of DNA damage, as opposed to being a repair gene.

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Documento generato il 30/09/20 alle ore 05:49:26