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Titolo:
Characterization of the effects of cannabinoids on guinea-pig tracheal smooth muscle tone: role in the modulation of acetylcholine release from parasympathetic nerves
Autore:
Spicuzza, L; Haddad, EB; Birrell, M; Ling, A; Clarke, D; Venkatesan, P; Barnes, PJ; Belvisi, MG;
Indirizzi:
Aventis Pharma Res & Dev, Dept Pharmacol, Dagenham RM10 7XS, Essex, England Aventis Pharma Res & Dev Dagenham Essex England RM10 7XS , Essex, England Univ London Imperial Coll Sci Technol & Med, Sch Med, Natl Heart & Lung Inst, Dept Thorac Med, London SW3 6LY, England Univ London Imperial Coll Sci Technol & Med London England SW3 6LY gland
Titolo Testata:
BRITISH JOURNAL OF PHARMACOLOGY
fascicolo: 7, volume: 130, anno: 2000,
pagine: 1720 - 1726
SICI:
0007-1188(200008)130:7<1720:COTEOC>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
RAT-BRAIN; RECEPTOR AGONIST; CB2 RECEPTORS; PHARMACOLOGY; EXPRESSION; INHIBITION; POTENT; DELTA-9-TETRAHYDROCANNABINOL; LOCALIZATION; ANTAGONIST;
Keywords:
cannabinoid; trachea; neurotransmission; acetylcholine release; contraction; airways;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
37
Recensione:
Indirizzi per estratti:
Indirizzo: Belvisi, MG Aventis Pharma Res & Dev, Dept Pharmacol, Rainham Rd S, Dagenham RM10 7XS,Essex, England Aventis Pharma Res & Dev Rainham Rd S Dagenham Essex England RM10 7XS
Citazione:
L. Spicuzza et al., "Characterization of the effects of cannabinoids on guinea-pig tracheal smooth muscle tone: role in the modulation of acetylcholine release from parasympathetic nerves", BR J PHARM, 130(7), 2000, pp. 1720-1726

Abstract

1 We investigated the ability of the cannabinoid agonists CP55,940 (CB1/CB2) and anandamide (endogenous cannabinoid) to modulate electrical field stimulation (EFS)-induced acetylcholine (ACh) release from parasympathetic nerve terminals innervating guinea-pig trachea. We assessed whether modulationof transmitter release translated to an impact on functional responses: byinvestigating the effect of these agents on contractile responses evoked by EFS and ACh. Furthermore, we evaluated the ability of these compounds to elicit bronchodilation in precontracted guinea-pig tracheal strips.2 CP55,940 and anandamide significantly inhibited EFS-evoked ACh release (maximal inhibition of 35.1+/-2.9% and 33.4+/-6.4% at 1 mu M, P<0.05, respectively). The CB1 receptor antagonist SR 141716A (1 mu M), had no effect on ACh release and failed to reverse the inhibitory effect of CP55,940 (1 mu M).3 Paradoxically, CP55,940 had no significant effect on EFS-evoked cholinergic contractile responses. Furthermore, CP55,940 did not relax pre-contracted tracheal strips or affect contractile responses to exogenous ACh. This lack of activity on smooth muscle tone is consistent with the fact that no detectable specific binding of [H-3] CP55,940 was found in tracheal homogenates.4 These data suggest that cannabinoid agonists inhibit ACh release from cholinergic nerve terminals via activation of CB2 receptors but that this inhibitory action does not impact on functional responses such as cholinergic contraction.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/12/20 alle ore 13:14:10