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Titolo:
Dopamine uptake by mouse neuroblastoma N1E-115 cells stably expressing human dopamine transporter is differentially inhibited by anti-idiotypic Ab2 beta antibodies mimicking the configuration of cocaine
Autore:
Ho, M; Segre, M;
Indirizzi:
Univ Illinois, Coll Vet Med, Dept Pathobiol, Urbana, IL 61802 USA Univ Illinois Urbana IL USA 61802 d, Dept Pathobiol, Urbana, IL 61802 USA
Titolo Testata:
BRAIN RESEARCH
fascicolo: 1-2, volume: 872, anno: 2000,
pagine: 231 - 235
SICI:
0006-8993(20000728)872:1-2<231:DUBMNN>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
NEUROTRANSMITTER TRANSPORTERS; NOREPINEPHRINE TRANSPORTERS; STRUCTURAL DOMAINS; BINDING; SUBSTRATE;
Keywords:
dopamine uptake; human dopamine transporter; cocaine; anti-idiotypic antibody; N1E-115; cocaine analog;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
19
Recensione:
Indirizzi per estratti:
Indirizzo: Segre, M Univ Illinois, Coll Vet Med, Dept Pathobiol, 2001 S Lincoln Ave, Urbana, IL 61802 USA Univ Illinois 2001 S Lincoln Ave Urbana IL USA 61802 IL 61802 USA
Citazione:
M. Ho e M. Segre, "Dopamine uptake by mouse neuroblastoma N1E-115 cells stably expressing human dopamine transporter is differentially inhibited by anti-idiotypic Ab2 beta antibodies mimicking the configuration of cocaine", BRAIN RES, 872(1-2), 2000, pp. 231-235

Abstract

We have successfully constructed a mouse neuronal N1E-115 cell line stablyexpressing a fully functional human dopamine transporter (hDAT). Previous studies in our laboratory have produced several anti-idiotypic Ab2 beta antibodies that mimic the configuration of the cocaine molecule at their antigen-combining site. In the present study, we observed that some anti-idiotypic Ab2 beta antibodies inhibited dopamine uptake by the hDAT-transfected line. Each antibody showed a different level of inhibition (between 40% and 90%). These findings suggest that the internal images of these antibodies can be used as analog peptides that may compete with cocaine for its binding site on the transporter but not impair dopamine uptake as much as cocaine does. (C) 2000 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/07/20 alle ore 12:23:55