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Titolo:
Estrogen modulates responses of striatal dopamine neurons to MPP+: evaluations using in vitro and in vivo techniques
Autore:
Arvin, M; Fedorkova, L; Disshon, KA; Dluzen, DE; Leipheimer, RE;
Indirizzi:
Youngstown State Univ, Dept Biol Sci, Youngstown, OH 44555 USA Youngstown State Univ Youngstown OH USA 44555 i, Youngstown, OH 44555 USA NE Ohio Univ, Coll Med, Dept Anat, Rootstown, OH 44272 USA NE Ohio Univ Rootstown OH USA 44272 d, Dept Anat, Rootstown, OH 44272 USA
Titolo Testata:
BRAIN RESEARCH
fascicolo: 1-2, volume: 872, anno: 2000,
pagine: 160 - 171
SICI:
0006-8993(20000728)872:1-2<160:EMROSD>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
MPTP-INDUCED NEUROTOXICITY; RAT DORSAL STRIATUM; SEX-DIFFERENCES; NUCLEUS-ACCUMBENS; ANESTHETIZED RAT; CD-1 MICE; RELEASE; ESTRADIOL; BRAIN; 17-BETA-ESTRADIOL;
Keywords:
electrochemistry; superfusion; transporter; neurotoxicity; nigrostriatal; neuroprotection;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
51
Recensione:
Indirizzi per estratti:
Indirizzo: Leipheimer, RE Youngstown State Univ, Dept Biol Sci, Youngstown, OH 44555 USA Youngstown State Univ Youngstown OH USA 44555 OH 44555 USA
Citazione:
M. Arvin et al., "Estrogen modulates responses of striatal dopamine neurons to MPP+: evaluations using in vitro and in vivo techniques", BRAIN RES, 872(1-2), 2000, pp. 160-171

Abstract

In vitro superfusion and in vivo electrochemistry were used to investigatethe role of estrogen in modulatings MPP+-induced dopamine output in the corpus striatum and nucleus accumbens of ovariectomized female rats. For in vitro superfusion experiments, dopamine and dihydroxyphenylacetic acid release were determined using HPLC with electrochemical detection from superfusion of corpus striatum fragments with Kreb's ringer phosphate buffer pulsed with MPP+ alone or MPP+ with estrogen. The in vivo electrochemistry experiments recorded the dopamine signal from carbon fiber microelectrodes stereotaxically passed through the corpus striatum and nucleus accumbens. Dopaminerelease was stimulated by pressure ejection of MPP+ alone or in combination with estrogen through glass micropipettes fastened to the electrodes. Dopamine output from superfusion chambers which received infusion of MPP+ withestrogen showed significantly lower output of dopamine compared with chambers which received MPP+ alone. Outputs of dihydroxyphenylacetic acid did not increase following MPP+ infusions. Data from the electrochemistry experiments demonstrated that estrogen significantly reduced both the amplitude and clearance rates of the MPP+-evokrd dopamine signal in both the corpus striatum and nucleus accumbens. Results of this study demonstrate that: (1) MPP+ evokes striatal dopamine release and this effect is significantly reduced in the presence of estrogen as determined by both in vivo electrochemistry and in vitro superfusion: (2) similar, albeit attenuated effects are observed in the nucleus accumbens as determined with in vivo electrochemistry; (3) estrogen acts to inhibit the clearance of dopamine in both the striatumand nucleus accumbens: and (4) estrogen may function as a neuroprotectant by reducing the uptake of neurotoxin into dopaminergic neurons. (C) 2000 Elsevier Science B.V. All rights reserved.

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Documento generato il 15/07/20 alle ore 08:38:54