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Titolo:
Involvement of calmodulin-dependent protein kinase II in carbachol-inducedrhythmic activity in the hippocampus of the rat
Autore:
Alberi, S; Boeijinga, PH; Raggenbass, M; Boddeke, HWGM;
Indirizzi:
Univ Groningen, Dept Med Physiol, NL-9712 KZ Groningen, Netherlands Univ Groningen Groningen Netherlands NL-9712 KZ Z Groningen, Netherlands Forenap, F-68250 Rouffach, France Forenap Rouffach France F-68250Forenap, F-68250 Rouffach, France CMU, Dept Physiol, Geneva, Switzerland CMU Geneva SwitzerlandCMU, Dept Physiol, Geneva, Switzerland Univ Groningen, Dept Med Physiol, NL-9712 KZ Groningen, Netherlands Univ Groningen Groningen Netherlands NL-9712 KZ Z Groningen, Netherlands
Titolo Testata:
BRAIN RESEARCH
fascicolo: 1-2, volume: 872, anno: 2000,
pagine: 11 - 19
SICI:
0006-8993(20000728)872:1-2<11:IOCPKI>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
LONG-TERM POTENTIATION; SYNAPTIC PLASTICITY; PYRAMIDAL CELLS; IN-VITRO; CHOLINERGIC EXCITATION; INTRACELLULAR CALCIUM; RECEPTOR SUBTYPES; POTASSIUM CURRENT; SLOW ACTIVITY; CA1 NEURONS;
Keywords:
carbachol; CaMKII; theta-like rhythm; rat hippocampal slice;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
49
Recensione:
Indirizzi per estratti:
Indirizzo: Boddeke, HWGM Univ Groningen, Dept Med Physiol, NL-9712 KZ Groningen, Netherlands Univ Groningen Groningen Netherlands NL-9712 KZ etherlands
Citazione:
S. Alberi et al., "Involvement of calmodulin-dependent protein kinase II in carbachol-inducedrhythmic activity in the hippocampus of the rat", BRAIN RES, 872(1-2), 2000, pp. 11-19

Abstract

The role of calcium and protein kinases in rhythmic activity induced by muscarinic receptor activation in the CA1 area in rat hippocampal slices was investigated. Extracellular recording showed that carbachol (20 mu M) induced synchronized field potential activity with a dominant frequency of 7.39+/-0.68 Hz. Pretreatment with the membrane permeable Ca2+ chelator BAPTA-AM (50 mu M) or with thapsigargin (1 mu M), a compound which depletes intracellular calcium stores, reduced the dominant power of carbachol-induced theta-like activity by 83% and 78%, respectively. Inhibition of calmodulin-dependent protein kinase II (CaMKII) by the cell permeable inhibitor KN-93 (10 mu M) reduced the power of carbachol-induced theta-like activity by 80%. In contrast the protein kinase C (PKC) inhibitor calphostin C did not significantly (P>0.05) affect the effect of carbachol. Whole-cell recording indicated that KN-93 also blocked carbachol-induced suppression of slow I-AHP and strongly inhibited the carbachol-induced plateau potential. Our data suggest that activation of CaMKII by carbachol is crucial for local theta-like activity in the CA1 area of the rat hippocampus in vitro. Furthermore, involvement of CaMKII in carbachol-induced suppression of the slow I-AHP and the induction of plateau potentials could play a role in the induction of theta-like rhythmic activity by carbachol. (C) 2000 Elsevier Science B.V. All rights reserved.

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Documento generato il 05/04/20 alle ore 03:10:36