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Titolo:
Antagonistic action of novel 1 alpha,25-dihydroxyvitamin D-3-26,23-lactoneanalogs on 25-hydroxyvitamin-D-3-24-hydroxylase gene expression induced by1 alpha,25-dihydroxyvitamin D-3 in human promyelocytic leukemia (HL-60) cells
Autore:
Ishizuka, S; Miura, D; Eguchi, H; Ozono, K; Chokki, M; Kamimura, T; Norman, AW;
Indirizzi:
Univ Calif Riverside, Dept Biochem, Riverside, CA 92521 USA Univ Calif Riverside Riverside CA USA 92521 chem, Riverside, CA 92521 USA Univ Calif Riverside, Div Biomed Sci, Riverside, CA 92521 USA Univ Calif Riverside Riverside CA USA 92521 Sci, Riverside, CA 92521 USA Res Inst Maternal & Child Hlth, Osaka 5941101, Japan Res Inst Maternal & Child Hlth Osaka Japan 5941101 Osaka 5941101, Japan Osaka Med Ctr, Dept Environm Med, Osaka 5941101, Japan Osaka Med Ctr Osaka Japan 5941101 ept Environm Med, Osaka 5941101, Japan Teijin Inst Biomed Res, Safety Res Dept, Hino, Tokyo 1918512, Japan TeijinInst Biomed Res Hino Tokyo Japan 1918512 ino, Tokyo 1918512, Japan Teijin Inst Biomed Res, Dept Bone & Calcium Metab, Hino, Tokyo 1918512, Japan Teijin Inst Biomed Res Hino Tokyo Japan 1918512 ino, Tokyo 1918512, Japan
Titolo Testata:
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
fascicolo: 1, volume: 380, anno: 2000,
pagine: 92 - 102
SICI:
0003-9861(20000801)380:1<92:AAON1A>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
VITAMIN-D-RECEPTOR; BIOLOGICAL-ACTIVITY ASSESSMENT; D-RESPONSIVE ELEMENTS; 1,25-DIHYDROXYVITAMIN D-3; 24-HYDROXYLASE GENE; NUCLEAR RECEPTOR; MONOCYTIC DIFFERENTIATION; 25-HYDROXYVITAMIN D-3; PROTEIN-KINASE; RAT-KIDNEY;
Keywords:
receptor; 1 alpha,25(OH)(2)D-3; vitamin D; antagonist; steroid metabolism;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
54
Recensione:
Indirizzi per estratti:
Indirizzo: Norman, AW Univ Calif Riverside, Dept Biochem, Riverside, CA 92521 USA Univ Calif Riverside Riverside CA USA 92521 side, CA 92521 USA
Citazione:
S. Ishizuka et al., "Antagonistic action of novel 1 alpha,25-dihydroxyvitamin D-3-26,23-lactoneanalogs on 25-hydroxyvitamin-D-3-24-hydroxylase gene expression induced by1 alpha,25-dihydroxyvitamin D-3 in human promyelocytic leukemia (HL-60) cells", ARCH BIOCH, 380(1), 2000, pp. 92-102

Abstract

We have demonstrated that 1 alpha,25-dihydroxyvitamin D-3-26,23-lactone analogs, (23S)- and (23R)-25-dehydro-1 alpha-hydroxyvitamin D-3-26,23-lactone(TEI-9647, TEI-9648, respectively), inhibit HL-60 cell differentiation induced by 1 alpha,25-dihydroxyvitamin D-2 [1 alpha;25(OH)(2)D-3], but not differentiation caused by all-trans retinoic acid (D, Miura ct at, 1999, J. Biol. Chem. 274, 16392), To assess whether the antagonistic actions of TEI-9647 and TEI-9648 in HL-60 cells are related to 1 alpha,25(OH)(2)D-3 breakdown, we investigated their effects on catabolism of 1 alpha,25(OH)(2)D-3. In HL-60 cells, the C-24 but not the C-23 side-chain oxidation pathway of 1 alpha,25(OH)(2)D-3 has been reported, Here we demonstrate that 1 alpha,25(OH)(2)D-3 was metabolized both to 24,25,26,27-tetranor-1 alpha,23-(OH)(2)D-3 and 1 alpha,25(OH)(2)D-3-26,23-lactone; thus HL-60 cells constitutively possess both the 24- and the 23-hydroxylases. Metabolism of 1 alpha,25(OH)(2)D-3 was strongly suppressed by 10(-7) M TEI-9647 or 10(-6) RI:TEI-9648. 1 alpha,25(OH)(2)D-3 alone slightly induced 24-hydroxylase gene expression by 8 h with full enhancement by 24-48 h; this induction was inhibited by 10(-6) M TEI-9647 and 10(-6) M TEI-9648 (86.2 and 31.9%, respectively) 24 h after treatment, However, analogs of TEI-9647 and TEI-9648 without the 25-dehydrofunctionality induced 24-hydroxylase gene expression. These results indicate that TEI-9647 and TEI-9648 clearly mediate their stereoselective antagonistic actions independent of their actions to block the catabolism of 1 alpha,25(OH)(2)D-3, Therefore, TEI-9647 and TEI-9648 appear to be the first antagonists specific for the nuclear 1 alpha,25(OH)(2)D-3 receptor-mediated genomic actions of 1 alpha,25(OH)(2)D-3 in HL-60 cells, (C)2000AcademicPress.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/09/20 alle ore 14:43:17