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Titolo:
Immunohistochemical localisation of protein tyrosine kinase receptors Tie-1 and Tie-2 in synovial tissue of rheumatoid arthritis: correlation with angiogenesis and synovial proliferation
Autore:
Uchida, T; Nakashima, M; Hirota, Y; Miyazaki, Y; Tsukazaki, T; Shindo, H;
Indirizzi:
Nagasaki Univ, Sch Med, Dept Anat 1, Nagasaki 8528523, Japan Nagasaki Univ Nagasaki Japan 8528523 ept Anat 1, Nagasaki 8528523, Japan Nagasaki Univ, Sch Med, Dept Orthopaed Surg, Nagasaki 8528523, Japan Nagasaki Univ Nagasaki Japan 8528523 opaed Surg, Nagasaki 8528523, Japan Nagasaki Univ, Sch Med, Atom Bomb Dis Inst, Dept Mol Pathol, Nagasaki 8528523, Japan Nagasaki Univ Nagasaki Japan 8528523 Mol Pathol, Nagasaki 8528523, Japan
Titolo Testata:
ANNALS OF THE RHEUMATIC DISEASES
fascicolo: 8, volume: 59, anno: 2000,
pagine: 607 - 614
SICI:
0003-4967(200008)59:8<607:ILOPTK>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
BLOOD-VESSEL FORMATION; ENDOTHELIAL-CELLS; STEM-CELLS; EXPRESSION; ANGIOPOIETIN-1; TEK; NEOVASCULARIZATION; VASCULOGENESIS; INFLAMMATION; MATURATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
30
Recensione:
Indirizzi per estratti:
Indirizzo: Tsukazaki, T Nagasaki Univ, Sch Med, Dept Anat 1, 1-12-4 Sakamoto, Nagasaki 8528523, Japan Nagasaki Univ 1-12-4 Sakamoto Nagasaki Japan 8528523 , Japan
Citazione:
T. Uchida et al., "Immunohistochemical localisation of protein tyrosine kinase receptors Tie-1 and Tie-2 in synovial tissue of rheumatoid arthritis: correlation with angiogenesis and synovial proliferation", ANN RHEUM D, 59(8), 2000, pp. 607-614

Abstract

Objective-To investigate the involvement of Tie-1 and Tie-2, receptor tyrosine kinases required for angiogenesis, in synovial proliferation and angiogenesis of rheumatoid arthritis (RA). Methods-Synovial tissues from 10 patients with RA and three control subjects were analysed by double immunohistochemistry and reverse transcriptase polymerase chain reaction (RT-PCR). Results-Expression of Tie-1 and Tie-2 was seen in all synovia, but predominantly in papillary projected portions. In synovial Lining cells, Tie-2 wasexpressed mainly in the basal layer and frequently colocalised with vimentin and proliferating cell nuclear antigen (PCNA), whereas Tie-1 was also expressed in the superficial layer. In stromal cells, Tie-2 immunoreactivity was restricted to vimentin positive fibroblast-but not macrophage derived cells, whereas Tie-1 expression was not dependent on the phenotype. Tie receptors were also highly expressed in the endothelium and surrounding pericytes of capillaries scattered over the papillary proliferated synovium without notable difference in the expression of the two receptors. Furthermore, Tie positive vessels often overexpressed PCNA. In normal synovia, expressionof Tie receptors was restricted to the capillary endothelium. RT-PCR confirmed the expression of Tie-1 and Tie-2 in RA synovial tissues and also in the cultured synoviocytes. Conclusion-The results suggest the possible involvement of overexpressed Tie-1 and Tie-2 in synovial lining and stromal cells in the pathophysiology of RA synovitis, probably through distinct mechanisms. Furthermore, expression of Tie receptors in actively growing vasculature may reflect the directinvolvement of these receptors in angiogenesis and subsequent vascularisation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/07/20 alle ore 07:23:31