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Titolo:
Free radical production in hypoxic pulmonary artery smooth muscle cells
Autore:
Killilea, DW; Hester, R; Balczon, R; Babal, P; Gillespie, MN;
Indirizzi:
Univ S Alabama, Coll Med, Dept Pharmacol, Mobile, AL 36688 USA Univ S Alabama Mobile AL USA 36688 , Dept Pharmacol, Mobile, AL 36688 USA Univ S Alabama, Coll Med, Dept Struct & Cellular Biol, Mobile, AL 36688 USA Univ S Alabama Mobile AL USA 36688 & Cellular Biol, Mobile, AL 36688 USA Univ S Alabama, Coll Med, Biotech Serv Lab, Mobile, AL 36688 USA Univ S Alabama Mobile AL USA 36688 Biotech Serv Lab, Mobile, AL 36688 USA
Titolo Testata:
AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY
fascicolo: 2, volume: 279, anno: 2000,
pagine: L408 - L412
SICI:
1040-0605(200008)279:2<L408:FRPIHP>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
REACTIVE OXYGEN; DIMETHYLTHIOUREA; PREVENTION; SCAVENGERS; INCREASES; PATHWAYS; THIOUREA; MYOCYTES; CALCIUM; INJURY;
Keywords:
fluorescence microscopy; hypoxia; reactive oxygen species; reactive nitrogen species; vascular smooth muscle cells;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
22
Recensione:
Indirizzi per estratti:
Indirizzo: Gillespie, MN Univ S Alabama, Coll Med, Dept Pharmacol, MSB 3130, Mobile, AL 36688 USA Univ S Alabama MSB 3130 Mobile AL USA 36688 le, AL 36688 USA
Citazione:
D.W. Killilea et al., "Free radical production in hypoxic pulmonary artery smooth muscle cells", AM J P-LUNG, 279(2), 2000, pp. L408-L412

Abstract

This study used an inexpensive and versatile environmental exposure systemto test the hypothesis that hypoxia promoted free radical production in primary cultures of rat main pulmonary artery smooth muscle cells (PASMCs). Production of reactive species was detected by fluorescence microscopy with the probe 2',7'-dichlorodihydrofluorescein, which is converted to the fluorescent dichlorofluorescein (DCF) in the presence of various oxidants. Flushing the airspace above the PASMC cultures with normoxic gas (20% O-2, 75% N-2, and 5% CO2) resulted in stable PO2 values of similar to 150 Torr, whereas perfusion of the airspace with hypoxic gas (0% O-2, 95% N-2, and 5% CO2)was associated with a reduction in PO2 values to stable levels of similar to 25 Torr. Hypoxic PASMCs became increasingly fluorescent at similar to 500% above the normoxic baseline after 60 min. Hypoxia-induced DCF fluorescence was attenuated by the addition of the antioxidants dimethylthiourea and catalase. These findings show that PASMCs acutely exposed to hypoxia exhibit a marked increase in intracellular DCF fluorescence, suggestive of reactive oxygen or nitrogen species production.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/11/20 alle ore 22:32:58