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Titolo:
Heparin-like molecules inhibit pulmonary vascular pericyte proliferation in vitro
Autore:
Khoury, J; Langleben, D;
Indirizzi:
McGill Univ, Sir Mortimer B Davis Jewish Gen Hosp, Div Cardiol, Montreal, PQ H3T 1E2, Canada McGill Univ Montreal PQ Canada H3T 1E2 diol, Montreal, PQ H3T 1E2, Canada McGill Univ, Sir Mortimer B Davis Jewish Gen Hosp, Lady Davis Inst Med Res, Montreal, PQ H3T 1E2, Canada McGill Univ Montreal PQ Canada H3T 1E2 Res, Montreal, PQ H3T 1E2, Canada
Titolo Testata:
AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY
fascicolo: 2, volume: 279, anno: 2000,
pagine: L252 - L261
SICI:
1040-0605(200008)279:2<L252:HMIPVP>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
SMOOTH-MUSCLE CELLS; PROTEIN-KINASE; FLAVOBACTERIUM-HEPARINUM; STRUCTURAL DETERMINANTS; IN-VITRO; CONTRACTILE PROTEINS; NA+/H+ EXCHANGE; LUNG PERICYTES; HYPERTENSION; GROWTH;
Keywords:
pulmonary hypertension; extracellular matrix; cell cycle; smooth muscle cell; proteoglycans;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
51
Recensione:
Indirizzi per estratti:
Indirizzo: Langleben, D McGill Univ, Sir Mortimer B Davis Jewish Gen Hosp, Div Cardiol, Room E258,3755 Cote St Catherine, Montreal, PQ H3T 1E2, Canada McGill Univ Room E258,3755 Cote St Catherine Montreal PQ Canada H3T 1E2
Citazione:
J. Khoury e D. Langleben, "Heparin-like molecules inhibit pulmonary vascular pericyte proliferation in vitro", AM J P-LUNG, 279(2), 2000, pp. L252-L261

Abstract

Proliferation of vascular pericytes (PCs), smooth muscle-like cells found in the distal microvasculature, contributes to vascular remodeling in pulmonary hypertension. The factors controlling lung PC quiescence in normal states are poorly understood. We demonstrate that exogenous heparin and heparan sulfate proteoglycans inhibit rat lung PC proliferation in vitro as does pulmonary vascular subendothelial matrix, particularly its heparan sulfate component. Heparin inhibits the intracellular alkalinization essential to proliferation, and we show that inhibition of alkalinization by 5-(N,N-dimethyl)amiloride also reduces PC proliferation. As shown by DNA staining and fluorescence-activated cell sorting analysis, heparin does not induce apoptosis in PCs. However, heparin maintains lung PCs in the G(0)/G(1) growth phase. Heparin induces production of p21, a potent inhibitor of cyclin-dependent kinases, thereby potentially identifying a fundamental mechanism by which heparin inhibits proliferation in smooth muscle-like cells. These studiesestablish additional similarities between lung PCs and smooth muscle cellsand provide further understanding of growth control in the lung microvasculature. They also further support the rationale that heparin-like moleculesmight be therapeutically beneficial in pulmonary hypertension.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/01/20 alle ore 20:26:14