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Titolo:
beta(1)-integrins are involved in migration of human fetal tracheal epithelial cells and tubular morphogenesis
Autore:
Coraux, C; Zahm, JM; Puchelle, E; Gaillard, D;
Indirizzi:
Univ Reims, CHU Maison Blanche, IFR 53, INSERM,U514, F-51092 Reims, FranceUniv Reims Reims France F-51092 R 53, INSERM,U514, F-51092 Reims, France
Titolo Testata:
AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY
fascicolo: 2, volume: 279, anno: 2000,
pagine: L224 - L234
SICI:
1040-0605(200008)279:2<L224:BAIIMO>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
BETA-1 INTEGRIN; BRANCHING MORPHOGENESIS; INTERCELLULAR-ADHESION; SUBMANDIBULAR-GLAND; LUNG DEVELOPMENT; EMBRYONIC LUNG; POTENTIAL ROLE; COLLAGEN GEL; IN-VITRO; PROLIFERATION;
Keywords:
cell-matrix interaction; collagen sandwich;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
37
Recensione:
Indirizzi per estratti:
Indirizzo: Gaillard, D Univ Reims, CHU Maison Blanche, IFR 53, INSERM,U514, 45 Rue Cognacq Jay, F-51092 Reims, France Univ Reims 45 Rue Cognacq Jay Reims France F-51092 ms, France
Citazione:
C. Coraux et al., "beta(1)-integrins are involved in migration of human fetal tracheal epithelial cells and tubular morphogenesis", AM J P-LUNG, 279(2), 2000, pp. L224-L234

Abstract

Development of human fetal airways requires interaction of the respiratoryepithelium and the extracellular matrix through integrins. Nevertheless, the specific roles of beta(1)-integrins during development and tubular morphogenesis are still unknown. To analyze beta(1)-integrin localization and influence during migration, we developed a model of human fetal tracheal explants growing on collagen and overlaid with a second layer of collagen to form a sandwich. In this configuration, cord and tubule formation proceeded normally but were inhibited by incubation with anti-beta(1)-integrin subunitantibodies. On a collagen matrix, beta(1)-integrins were immunolocalized on the entire plasma membrane of migrating epithelial cells and almost exclusively on the basal plasma membrane of nonmigratory epithelial cells. In a sandwich configuration, beta(1)-integrins became detectable in the cytoplasm of epithelial cells. Coating cultures with collagen transiently altered the morphology of migrating cells and their speed and direction of migration, whereas incubation with anti-beta(1)-integrin subunit antibodies irreversibly altered these parameters. These observations suggest that the matrix environment, by modulating beta(1)-integrin expression patterns, plays a keyrole during tubular morphogenesis of human fetal tracheal epithelium, principally by modulating epithelial cell migration.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 14/08/20 alle ore 15:25:13