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Titolo:
In situ monitoring of natural genetic transformation of Acinetobacter calcoaceticus BD413 in monoculture biofilms
Autore:
Hendrickx, L; Hausner, M; Wuertz, S;
Indirizzi:
Tech Univ Munich, Inst Water Qual Control & Waste Management, D-85748 Garching, Germany Tech Univ Munich Garching Germany D-85748 ent, D-85748 Garching, Germany
Titolo Testata:
WATER SCIENCE AND TECHNOLOGY
fascicolo: 4-5, volume: 41, anno: 2000,
pagine: 155 - 158
SICI:
0273-1223(2000)41:4-5<155:ISMONG>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Keywords:
Acinetobacter calcoaceticus; biofilm; confocal laser scanning microscopy; horizontal gene transfer; in situ genetic engineering; natural genetic transformation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
5
Recensione:
Indirizzi per estratti:
Indirizzo: Hendrickx, L Tech Univ Munich, Inst Water Qual Control & Waste Management,D-85748 Garching, Germany Tech Univ Munich Garching Germany D-85748 Garching, Germany
Citazione:
L. Hendrickx et al., "In situ monitoring of natural genetic transformation of Acinetobacter calcoaceticus BD413 in monoculture biofilms", WATER SCI T, 41(4-5), 2000, pp. 155-158

Abstract

Confocal laser scanning microscopy (CLSM) was used for the detection of natural genetic transformation in a defined pure culture Acinetobacter calcoaceticus BD413 biofilm, with the enhanced yellow fluorescent protein (EYFP) as a nonselective genetic marker. The effect of free DNA concentration and of exposure time to free DNA on transformation frequency was explored. Transformation studies indicated that Acinetobacter calcoaceticus BD413 cells exposed to as little as 0.1 ng free DNA/ml resulted in detectable transformation frequencies (1x10(-4) transformed cells/recipient cell). Within 2 hours about 20% of the biofilm cells expressed eyfp after exposure to 1 mu g free DNA/ml. Microscopic analysis revealed a unique distribution of transformation frequencies in the biofilm. The highest frequencies were found near the substratum or near the biofilm/medium interface.

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Documento generato il 28/03/20 alle ore 23:23:46