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Titolo:
Human immunodeficiency virus type 1 Nef epitopes recognized in HLA-A2 transgenic mice in response to DNA and peptide immunization
Autore:
Sandberg, JK; Leandersson, AC; Devito, C; Kohleisen, B; Erfle, V; Achour, A; Levi, M; Schwartz, S; Karre, K; Wahren, B; Hinkula, J;
Indirizzi:
Karolinska Inst, Ctr Microbiol & Tumor Biol, S-17177 Stockholm, Sweden Karolinska Inst Stockholm Sweden S-17177 Biol, S-17177 Stockholm, Sweden Swedish Inst Infect Dis Control, Dept Virol, S-17182 Stockholm, Sweden Swedish Inst Infect Dis Control Stockholm Sweden S-17182 ockholm, Sweden Forsch Zentrum Neuberg, Inst Mol Virol, D-85764 Oberschleissheim, Germany Forsch Zentrum Neuberg Oberschleissheim Germany D-85764 issheim, Germany Uppsala Univ, Dept Microbiol, Uppsala, Sweden Uppsala Univ Uppsala Sweden psala Univ, Dept Microbiol, Uppsala, Sweden
Titolo Testata:
VIROLOGY
fascicolo: 1, volume: 273, anno: 2000,
pagine: 112 - 119
SICI:
0042-6822(20000720)273:1<112:HIVT1N>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
CYTOTOXIC T-LYMPHOCYTES; MAJOR HISTOCOMPATIBILITY COMPLEX; CLASS-I MOLECULES; LONG-TERM SURVIVOR; MHC CLASS-I; HIV-1 NEF; IMMUNE-RESPONSES; GENETIC-VARIATION; CELLS; INFECTION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
54
Recensione:
Indirizzi per estratti:
Indirizzo: Sandberg, JK Rockefeller Univ, AIDS Res Ctr, 455 1st Ave,7th Floor, New York, NY 10016 USA Rockefeller Univ 455 1st Ave,7th Floor New York NY USA 10016
Citazione:
J.K. Sandberg et al., "Human immunodeficiency virus type 1 Nef epitopes recognized in HLA-A2 transgenic mice in response to DNA and peptide immunization", VIROLOGY, 273(1), 2000, pp. 112-119

Abstract

We investigated the immune response against a human immunodeficiency Virustype I (HIV-1) nef DNA sequence administered epidermally in mice transgenic for the human major histocompatibility complex (MHC) class I molecule HLA-A201. Ten potential HLA-AP binding 9-mer Nef peptides were identified by acomputer-based search algorithm. By a cell surface MHC class I stabilization assay, four peptides were scored as good binders, whereas two peptides bound weakly to HLA-AP. After DNA immunization. cytotoxic T lymphocyte (CTL)responses were predominantly directed against the Nef 44-52, 81-89, and 85-93 peptides. Interestingly. the 44-52 epitope resides outside the regions of Nef where previously described CTL epitopes are clustered. Dominance among Nef-derived peptides did not strictly correlate with HLA-A2 binding, in that only one of the high-affinity binding peptides was targeted in the CTLresponse. The 44-52, 85-93, and 139-147 peptides also generated specific CTLs in response to peptide immunization. T helper cell proliferation was detected after stimulation with 20-mer peptides in vitro. Three Nef regions (16-35, 106-125, and 166-185) dominated the T helper cell proliferation. Theimplications of these results for the development of DNA-based vaccines against HIV is discussed. (C) 2000 Academic Press.

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Documento generato il 28/03/20 alle ore 10:09:28