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Titolo:
Melanomas and melanoma cell lines do not express HLA-G, and the expressioncannot be induced by gamma IFN treatment
Autore:
Frumento, G; Franchello, S; Palmisano, GL; Nicotra, MR; Giacomini, P; Loke, YW; Geraghty, DE; Maio, M; Manzo, C; Natali, PG; Ferrara, GB;
Indirizzi:
IST Natl Inst Canc Res, Immunogenet Lab, Genoa, Italy IST Natl Inst Canc Res Genoa Italy c Res, Immunogenet Lab, Genoa, Italy Regina Elena Inst CRS, Immunol Lab, Rome, Italy Regina Elena Inst CRS Rome Italy ena Inst CRS, Immunol Lab, Rome, Italy Natl Res Council, Inst Biomed Technol, Rome, Italy Natl Res Council RomeItaly s Council, Inst Biomed Technol, Rome, Italy Univ Cambridge, Cambridge, England Univ Cambridge Cambridge EnglandUniv Cambridge, Cambridge, England Fred Hutchinson Canc Res Ctr, Human Immunogenet Program, Seattle, WA 98104USA Fred Hutchinson Canc Res Ctr Seattle WA USA 98104 m, Seattle, WA 98104USA CRO, Alternat Immunotherapy Unit, Aviano, Italy CRO Aviano ItalyCRO, Alternat Immunotherapy Unit, Aviano, Italy G Pascale Fdn, Naples, Italy G Pascale Fdn Naples ItalyG Pascale Fdn, Naples, Italy Univ Genoa, SMFN, Genoa, Italy Univ Genoa Genoa ItalyUniv Genoa, SMFN, Genoa, Italy
Titolo Testata:
TISSUE ANTIGENS
fascicolo: 1, volume: 56, anno: 2000,
pagine: 30 - 37
SICI:
0001-2815(200007)56:1<30:MAMCLD>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
NATURAL-KILLER-CELLS; NK CELLS; MONOCLONAL-ANTIBODIES; PROTEIN EXPRESSION; HUMAN TUMORS; IMMUNOSURVEILLANCE; TRANSCRIPTION; MOLECULES; RECEPTORS; ANTIGEN;
Keywords:
HLA-G; interferon; melanoma; nevus;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
24
Recensione:
Indirizzi per estratti:
Indirizzo: Ferrara, GB Adv Biotechnol Ctr, Immunogenet Lab, CBA A2, Largo Rosanna Benzi 10, I-16132 Genoa, Italy Adv Biotechnol Ctr Largo Rosanna Benzi 10 Genoa Italy I-16132
Citazione:
G. Frumento et al., "Melanomas and melanoma cell lines do not express HLA-G, and the expressioncannot be induced by gamma IFN treatment", TISSUE ANTI, 56(1), 2000, pp. 30-37

Abstract

HLA-G is an effective ligand of natural killer (NK) inhibitory receptors, HLA-G transcripts have been detected in several human tumors, and cytokineslike gamma interferon (IFN) enable HLA-G molecules to be expressed. These findings are particularly upsetting in case of melanomas: IFN treatment is frequently included in melanoma therapeutic protocols, and downregulation of classical class I molecules occurs in nearly half of these tumors Therefore, a melanoma cell downregulating classical class I and de novo expressingHLA-G, either constitutively or upon IFN treatment, is probably a stealthytarget for the immune system, having inhibited both the cytotoxic T lymphocyte (CTL) and the NK activity. To elucidate this point we have investigated the expression of HLA-G molecules in 45 melanoma cell lines before and after gamma IFN treatment. Analysis was performed by immunofluorescence and flow cytometry, using the anti-HLA-G MoAbs 87G and G233, by Western blot, using the anti-HLA-G MEM/G1 MoAb and PAG1 antiserum, and by RT-PCR analysis In addition, 8 melanoma tissues from patients free from therapy and 6 nevi were studied by immunohistochemistry using the 87G MoAb. No evidence was gathered of HLA-G expression, neither constitutive nor, in cell lines, after gamma IFN treatment. We therefore conclude that HLA-G expression is an uncommon event in melanomas, and that a therapy including IFNs cannot harm the patient by inducing the de novo expression of HLA-G molecules at least in its G1 isoform.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 11/07/20 alle ore 07:33:21