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Titolo:
Microsatellite analysis of Aegilops tauschii germplasm
Autore:
Pestsova, E; Korzun, V; Goncharov, NP; Hammer, K; Ganal, MW; Roder, MS;
Indirizzi:
IPK, D-06466 Gatersleben, Germany IPK Gatersleben Germany D-06466IPK, D-06466 Gatersleben, Germany Russian Acad Sci, Inst Cytol & Genet, Novosibirsk 630090, Russia Russian Acad Sci Novosibirsk Russia 630090 t, Novosibirsk 630090, Russia Univ Gesamthsch Kassel, D-37213 Witzenhausen, Germany Univ Gesamthsch Kassel Witzenhausen Germany D-37213 itzenhausen, Germany
Titolo Testata:
THEORETICAL AND APPLIED GENETICS
fascicolo: 1-2, volume: 101, anno: 2000,
pagine: 100 - 106
SICI:
0040-5752(200007)101:1-2<100:MAOATG>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
D-GENOME; TRITICUM-TAUSCHII; GENETIC DIVERSITY; HEXAPLOID WHEAT; MARKERS; ACCESSIONS; MAP;
Keywords:
Aegilops tauschii; D genome; microsatellite markers; genetic diversity; germplasm;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
20
Recensione:
Indirizzi per estratti:
Indirizzo: Pestsova, E IPK, Corrensstr 3, D-06466 Gatersleben, Germany IPK Corrensstr3 Gatersleben Germany D-06466 rsleben, Germany
Citazione:
E. Pestsova et al., "Microsatellite analysis of Aegilops tauschii germplasm", THEOR A GEN, 101(1-2), 2000, pp. 100-106

Abstract

The highly polymorphic diploid grass Aegilogs tauschii is the D-genome donor to hexaploid wheat and represents a potential source for bread wheat improvement. In the present study microsatellite markers were used for germplasm analysis and estimation of the genetic relationship between 113 accessions of Ae. tauschii From the gene bank collection at IPK, Gatersleben. Eighteen microsatellite markers, developed from Triticum aestivum and Ae. tauschii sequences, were selected for the analysis. All microsatellite markers showed a high level of polymorphism. The number of alleles per microsatellitemarker varied from 11 to 25 and a total of 338 alleles were detected. The number of alleles per locus in cultivated bread wheat germplasm had previously been found to be significantly lower. The highest levels of genetic diversity for microsatellite markers were found in accessions from the Caucasian countries (Georgia, Armenia and the Daghestan region of Russia) and the lowest in accessions from the Central Asian countries (Uzbekistan and Turkmenistan). Genetic dissimilarity values between accessions were used to produce a dendrogram of the relationships among the accessions. The result showed that all of the accessions could be distinguished and clustered into twolarge groups in accordance with their subspecies taxonomic classification. The pattern of clustering of the Ae. tauschii accessions is according to their geographic distribution. The data suggest that a relatively small number of microsatellites can be used to estimate genetic diversity in the germplasm of Ae. tauschii and confirm the good suitability of microsatellite markers for the analysis of germplasm collections.

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Documento generato il 22/10/20 alle ore 02:57:57