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Titolo:
Laser-induced fluorescence detection of malignant gliomas using fluorescein-labeled serum albumin: Experimental and preliminary clinical results
Autore:
Kremer, P; Wunder, A; Sinn, H; Haase, T; Rheinwald, M; Zillmann, U; Albert, FK; Kunze, S;
Indirizzi:
Univ Heidelberg, Dept Neurosurg, Kopfklinikum, Heidelberg, Germany Univ Heidelberg Heidelberg Germany g, Kopfklinikum, Heidelberg, Germany German Canc Res Ctr, D-6900 Heidelberg, Germany German Canc Res Ctr Heidelberg Germany D-6900 D-6900 Heidelberg, Germany Paracelsus Klin, Dept Neurosurg, Osnabruck, Germany Paracelsus Klin Osnabruck Germany n, Dept Neurosurg, Osnabruck, Germany
Titolo Testata:
NEUROLOGICAL RESEARCH
fascicolo: 5, volume: 22, anno: 2000,
pagine: 481 - 489
SICI:
0161-6412(200007)22:5<481:LFDOMG>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
ENHANCED TUMOR UPTAKE; PORPHYRIN FLUORESCENCE; PHOTODYNAMIC THERAPY; RESIDUAL TUMOR; BRAIN-TUMORS; RAT GLIOMA; MARGINS; RESECTION; INVIVO; DEGRADATION;
Keywords:
fluorescein; fluorescence detection; glioma; albumin;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
39
Recensione:
Indirizzi per estratti:
Indirizzo: Kremer, P Univ Heidelberg, Neurochirurg Klin, Neuenheimer Feld 400, D-69120 Heidelberg, Germany Univ Heidelberg Neuenheimer Feld 400 Heidelberg Germany D-69120
Citazione:
P. Kremer et al., "Laser-induced fluorescence detection of malignant gliomas using fluorescein-labeled serum albumin: Experimental and preliminary clinical results", NEUROL RES, 22(5), 2000, pp. 481-489

Abstract

To delineate the tumor margins of malignant gliomas laser-induced fluorescence detection technique was applied using 5-aminofluorescein-albumin as the fluorescent dye. The 5-aminofluorescein was linked to serum albumin (= AFlc-SA) as a cumulative protein label using residualizing markers. In a CG-glioma model the biodistribution and pharmacokinetics of the injected dye were investigated by labeling the protein conjugate with (111)ln-DTPA. Twenty-four hours after intravenous injection of the dye, fluorescence was activated by an argon laser and inspected in the C6-gliomas. Histological examinations were performed to compare the microscopic margins of the fluorescence-stained tumors with hematoxylin/eosin. The tumor uptake 24 h after dye injection was 23-fold higher than in the surrounding brain. Fluorescence inspection under laser activation demonstrated clearly stained and sharply demarcated tumors. The microscopic borders of the tumors corresponded exactly with the fluorescence, also demonstrating intracellular tumor uptake of the dye. In a preliminary study, three patients with malignant gliomas were operated using laser-induced fluorescence detection technique after injection of AFlc-SA. In all patients, the borders of the malignant gliomas were clearly stained by AFlc-SA during surgery. Laser-induced fluorescence imaging using the albumin conjugate AFlc-SA may be a promising method for delineatingtumor margins which are hard to detect under the operating microscope alone.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/01/21 alle ore 14:12:29