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Titolo:
BCR-ABL prevents c-Jun-mediated and proteasome-dependent FUS (TLS) proteolysis through a protein kinase C beta II-dependent pathway
Autore:
Perrotti, D; Iervolino, A; Cesi, V; Cirinna, M; Lombardini, S; Grassilli, E; Bonatti, S; Claudio, PP; Calabretta, B;
Indirizzi:
Thomas Jefferson Univ, Kimmel Canc Ctr, Dept Microbiol & Immunol, Philadelphia, PA 19107 USA Thomas Jefferson Univ Philadelphia PA USA 19107 hiladelphia, PA 19107 USA Univ Modena, Dept Biomed Sci, Sect Gen Pathol, I-41100 Modena, Italy Univ Modena Modena Italy I-41100 Sect Gen Pathol, I-41100 Modena, Italy
Titolo Testata:
MOLECULAR AND CELLULAR BIOLOGY
fascicolo: 16, volume: 20, anno: 2000,
pagine: 6159 - 6169
SICI:
0270-7306(200008)20:16<6159:BPCAPF>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
RNA-BINDING PROTEINS; ORNITHINE DECARBOXYLASE; TYROSINE KINASE; V-ABL; ONCOGENIC TRANSFORMATION; DELTA-DOMAIN; MAP KINASES; DEGRADATION; UBIQUITIN; TRANSCRIPTION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
43
Recensione:
Indirizzi per estratti:
Indirizzo: Perrotti, D Thomas Jefferson Univ, Kimmel Canc Ctr, Dept Microbiol & Immunol, Blsb 630,233 S 10th St, Philadelphia, PA 19107 USA Thomas Jefferson Univ Blsb 630,233 S 10th St Philadelphia PA USA 19107
Citazione:
D. Perrotti et al., "BCR-ABL prevents c-Jun-mediated and proteasome-dependent FUS (TLS) proteolysis through a protein kinase C beta II-dependent pathway", MOL CELL B, 20(16), 2000, pp. 6159-6169

Abstract

The DNA binding activity of FUS (also known as TLS), a nuclear pro-oncogene involved in multiple translocations, is regulated by BCR-ABL in a proteinkinase cpn (PKC beta II)-dependent manner. We show here that in normal myeloid progenitor cells FUS, although not visibly ubiquitinated, undergoes proteasome-dependent degradation, whereas in BCR-ABL-expressing cells, degradation is suppressed by PKC beta II phosphorylation. Replacement of serine 256 with the phosphomimetic aspartic acid prevents proteasome-dependent proteolysis of FUS, while the serine-256-to-alanine FUS mutant is unstable and susceptible to degradation. Ectopic expression of the phosphomimetic S256D FUS mutant in granulocyte colony-stimulating factor-treated 32Dcl3 cells induces massive apoptosis and inhibits the differentiation of the cells escaping cell death, while the degradation-prone S256A mutant has no effect on either survival or differentiation. FUS proteolysis is induced by c-Jun, is suppressed by BCR-ABL or Jun kinase 1, and does not depend on c-Jun transactivation potential, ubiquitination, or its interaction with Jun kinase 1, In addition, c-Jun induced FUS proteasome-dependent degradation is enhanced by heterogeneous nuclear ribonucleoprotein (hnRNP) A1 and depends on the formation of a FUS-Jun-hnRNP A1-containing complex and on lack of PKC beta IIphosphorylation at serine 256 but not on FUS ubiquitination. Thus, novel mechanisms appear to be involved in the degradation of FUS in normal myeloidcells; moreover, the ability of the BCR-ABL oncoprotein to suppress FUS degradation by the induction of posttranslational modifications might contribute to the phenotype of BCR-ABL expressing hematopoietic cells.

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Documento generato il 01/10/20 alle ore 07:20:35