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Titolo:
Bradykinin and nitric oxide generation by dialysis membranes can be blunted by alkaline rinsing solutions
Autore:
Coppo, R; Amore, A; Cirina, P; Scelfo, B; Giacchino, F; Comune, L; Atti, M; Renaux, JL;
Indirizzi:
Osped Regina Margherita, Div Nefrol & Dialisi, I-10126 Turin, Italy Osped Regina Margherita Turin Italy I-10126 ialisi, I-10126 Turin, Italy Osped Civile, Nephrol & Dialysis Dept, Ivrea, Italy Osped Civile Ivrea Italy Civile, Nephrol & Dialysis Dept, Ivrea, Italy Hosp SpA, Bologna, Italy Hosp SpA Bologna ItalyHosp SpA, Bologna, Italy Hosp R&D Int, Lyon, France Hosp R&D Int Lyon FranceHosp R&D Int, Lyon, France
Titolo Testata:
KIDNEY INTERNATIONAL
fascicolo: 2, volume: 58, anno: 2000,
pagine: 881 - 888
SICI:
0085-2538(200008)58:2<881:BANOGB>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
ANAPHYLACTOID REACTIONS; HYPERSENSITIVITY REACTIONS; ACE INHIBITORS; AN69 MEMBRANES; HEMODIALYSIS; ACTIVATION; KALLIKREIN;
Keywords:
hypersensitivity reactions; bio-incompatibility; nitric oxide; filter rinsing solutions; hemodialysis; AN69;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
30
Recensione:
Indirizzi per estratti:
Indirizzo: Coppo, R Osped Regina Margherita, Div Nefrol & Dialisi, Piazza Polonia 94,I-10126 Turin, Italy Osped Regina Margherita Piazza Polonia 94 Turin Italy I-10126 ly
Citazione:
R. Coppo et al., "Bradykinin and nitric oxide generation by dialysis membranes can be blunted by alkaline rinsing solutions", KIDNEY INT, 58(2), 2000, pp. 881-888

Abstract

Background Bradykinin(BK) generation following the first contact of blood with the dialysis materials is thought to enhance hypersensitivity reactions (HSRs). Some of the effects of BK are mediated by nitric oxide (NO). We have recently reported that the pH of diluted blood modulates the kinin system. The present study was aimed to investigate the role of the pH of culture media and filter-washing solutions and BK and NO generation, either in vitro and ex vivo. Methods. BK was measured by a specific enzyme-linked immunosorbent assay (ELISA), and NO synthase (NOS) activity by H-3-citrulline production after incubation with H-3-arginine and nitrites by using the Griess reagent. In invitro experiments, NOS activity was detected in endothelial cells (ECs) cultured with graded BK concentrations at various pH values. Blood from 30 patients in regular dialysis was ex vivo circulated in one single passage through minifilters prerinsed with pH 7 or pH 8 phosphate buffer (PB) solutions. The out-flowing blood was tested for BK and nitrite content and was incubated with cultured ECs to evaluate its capacity to modulate NOS activity. Results. BK induced in vitro a dose-dependent increase in NOS activity of ECs, which was mediated by tyrosine kinase phosphorylation. NO generation was enhanced at pH 7.2, which remained unchanged at pH 7.6. In ex vivo experiments. blood out-flowing after one passage on filters washed with pH 7 PB solutions had increased BK levels (P < 0.0001), increased nitrites (P < 0.05), and enhanced EC NOS activity (P < 0.05) in comparison to data found when filters were washed with pH 8 PB. Only when the filters were rinsed with a solution at pH 7 did PAN DX and AN69 membranes show a distinct BK generation capability, and cuprophane a peculiar capability to enhance NOS. Such effects were prevented when dialyzers were prerinsed with pH 8 PB. Multiple regression analysis showed that the pH of the uremic blood was the driving factor for BK and NOS activation (r = 0.54, P < 0.02). Conclusions. BK and NO generation are modulated by environmental pH. Rinsing the blood and dialysate compartments of filters with an alkaline solution prior to use may mitigate the activation of mediators likely to be involved in some HSRs.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/01/21 alle ore 03:58:20