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Titolo:
Mesangial factor V expression colocalized with fibrin deposition in IgA nephropathy
Autore:
Liu, N; Ono, T; Suyama, K; Nogaki, F; Shirakawa, K; Maeda, M; Kawamura, T; Kamata, T; Oyama, A; Muso, E; Sasayama, S;
Indirizzi:
Kyoto Univ, Grad Sch Med, Dept Cardiovasc Med, Sakyo Ku, Kyoto 6068507, Japan Kyoto Univ Kyoto Japan 6068507 ovasc Med, Sakyo Ku, Kyoto 6068507, Japan
Titolo Testata:
KIDNEY INTERNATIONAL
fascicolo: 2, volume: 58, anno: 2000,
pagine: 598 - 606
SICI:
0085-2538(200008)58:2<598:MFVECW>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN GLOMERULAR-DISEASES; FACTOR PATHWAY INHIBITOR; CROSS-LINKED FIBRIN; CRESCENTIC GLOMERULONEPHRITIS; COAGULATION FACTOR; BIOSYNTHESIS; LOCALIZATION; NEPHRITIS; THROMBIN; RABBITS;
Keywords:
glomerulonephritis; renal biopsy; cross-linked fibrin; inflammatory response; alpha-smooth muscle actin;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
33
Recensione:
Indirizzi per estratti:
Indirizzo: Ono, T Kyoto Univ, Grad Sch Med, Dept Cardiovasc Med, Sakyo Ku, 54 ShogoinKawaracho, Kyoto 6068507, Japan Kyoto Univ 54 Shogoin Kawaracho Kyoto Japan 6068507 6068507, Japan
Citazione:
N. Liu et al., "Mesangial factor V expression colocalized with fibrin deposition in IgA nephropathy", KIDNEY INT, 58(2), 2000, pp. 598-606

Abstract

Background. Factor V in its active form (Va) plays a key role at the termination of the intrinsic coagulation pathway, serving as a membrane-bound cofactor for the conversion of prothrombin to thrombin by factor Xa. Cross-linked fibrin (XFb) is often observed in mesangial areas in active types of human glomerulonephritis. In this study, to clarify contribution of factor Vin intramesangial coagulation. mesangial factor V expression and its relationship to mesangial proliferation and fibrin deposition in IgA nephropathy(IgAN) were investigated. Methods. Twenty-two patients with IgAN were studied. XPb was detected in renal biopsy specimens using anti-d-dimer antibody combined with plasmin exposure, and factor V was detected with rabbit antibody against human factor V. Double-labeling immunohistochemistry was used to investigate the relationship of the glomerular distribution of factor V to XFb. The relationship of factor V staining to the activity index or XFb, deposition was evaluated. The expression of factor V mRNA was assessed by in situ hybridization in relationship to the antigen staining of or-smooth muscle actin (alpha-SMA). The ultrastructural distribution of factor V in glomeruli was studied by immunoelectron microscopy. Results. XFb and factor V were observed in the mesangium and along capillary loops in seven and nine specimens. respectively. Factor V had intense, frequent expression in the proliferating and necrotizing areas, showing a significant relationship to XFb (P < 0.05). Furthermore. XFb deposition and factor V expression were markedly correlated with disease activity (P = 0.005 and P = 0.008, respectively). By double-labeling experiments. XFb and factor V were often seen colocalized in mesangial areas of the glomeruli, which showed necrotizing lesions and/or intense cellular proliferation. By in situ hybridization. factor V mRNA was detected mainly in the mesangial cells, which were positive for alpha-SMA, and partly in the endothelial cells. By immunoelectron microscopy, factor V presence was confirmed in the mesangium and endothelium. Conclusion. The present findings suggest that factor V is strongly expressed in mesangial cells in active IgAN accompanied with mesangial proliferation and may exert procoagulant activity, leading to intramesangial coagulation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/04/20 alle ore 05:43:47