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Titolo:
Absorption of milk-borne insulin-like growth factor-I into portal blood ofsuckling rats
Autore:
Philipps, AF; Dvorak, B; Kling, PJ; Grille, JG; Koldovsky, O;
Indirizzi:
Univ Calif Davis, Dept Pediat, Sacramento, CA 95817 USA Univ Calif Davis Sacramento CA USA 95817 Pediat, Sacramento, CA 95817 USA Univ Arizona, Coll Med, Steel Mem Childrens Res Ctr, Furrow Res lab, Tucson, AZ 85724 USA Univ Arizona Tucson AZ USA 85724 tr, Furrow Res lab, Tucson, AZ 85724 USA
Titolo Testata:
JOURNAL OF PEDIATRIC GASTROENTEROLOGY AND NUTRITION
fascicolo: 2, volume: 31, anno: 2000,
pagine: 128 - 135
SICI:
0277-2116(200008)31:2<128:AOMIGF>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
FACTOR BINDING-PROTEINS; MESSENGER-RNA; GASTROINTESTINAL-TRACT; IGF-I; RECEPTORS; PIGS;
Keywords:
insulin-like growth factor; milk; nutrition; portal venous blood; suckling;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
29
Recensione:
Indirizzi per estratti:
Indirizzo: Philipps, AF Univ Calif Davis, Dept Pediat, 2516 Stockton Blvd,3rd Floor, Sacramento, CA 95817 USA Univ Calif Davis 2516 Stockton Blvd,3rd Floor Sacramento CA USA 95817
Citazione:
A.F. Philipps et al., "Absorption of milk-borne insulin-like growth factor-I into portal blood ofsuckling rats", J PED GASTR, 31(2), 2000, pp. 128-135

Abstract

Background: Insulin-like growth factors (IGFs) are potent mitogens that have been implicated in control of growth and development during the perinatal period. These hormones are also present in biologically significant quantities in mammalian milks. Although one site of action of these IGFs may be at the intestinal level, current information about whether they pass intactinto the circulation is conflicting. Methods: To test the hypothesis that milk-borne IGFs an absorbed into blood in receptor-active form, suckling rats were given either recombinant human (rh)I-125-IGF-I or -II (4 x 10(6) counts per minute [cpm]), and the activity present in portal and cardiac blood was examined at 5, 10, 20, and 30 minutes after ingestion for presence of appropriate molecular weight peptides in these samples. Tn selected samples, purified radioactive samples were tested for their ability to bind competitively to crude membranes bearing IGF receptors. Results: The results of these studies indicate that rh(125)I-IGF-I is absorbed in receptor-active form into the portal circulation and that maximal amounts are present 10 to 30 minutes after ingestion. Estimation of the presence of intact hormone was made on the basis of the elution profile of samples when run on gel chromatography as well as reversed-phase high-performance liquid chromatography. Isolated samples from portal blood also bound competitively to placental membranes bearing IGF receptors. In contrast, rh(125)I-IGF-II could not be demonstrated in receptor-active form in portal blood. Chromatography showed appropriate sized peaks with greater activity in portal than cardiac samples, but competitive binding was not appreciated. Conclusions: It is likely that at least milk-home IGF-I is absorbed intactand may exert effects on liver and other peripheral tissues. In addition, this study lends further credence to the possibility of an enterohepatic circulation for IGF-I.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/09/20 alle ore 07:50:14