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Titolo:
Higher HIV-1 RNA cutoff level required in cerebrospinal fluid than in blood to predict positive HIV-1 isolation
Autore:
Andersson, LM; Svennerholm, B; Hagberg, L; Gisslen, M;
Indirizzi:
Gothenburg Univ, Dept Infect Dis, Inst Internal Med, Sahlgrenska Univ Hosp, S-41685 Gothenburg, Sweden Gothenburg Univ Gothenburg Sweden S-41685 sp, S-41685 Gothenburg, Sweden Gothenburg Univ, Dept Clin Virol, Sahlgrenska Univ Hosp, S-41346 Gothenburg, Sweden Gothenburg Univ Gothenburg Sweden S-41346 sp, S-41346 Gothenburg, Sweden
Titolo Testata:
JOURNAL OF MEDICAL VIROLOGY
fascicolo: 1, volume: 62, anno: 2000,
pagine: 9 - 13
SICI:
0146-6615(200009)62:1<9:HHRCLR>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN-IMMUNODEFICIENCY-VIRUS; AIDS DEMENTIA COMPLEX; ACTIVE ANTIRETROVIRAL THERAPY; VIRAL LOAD; NEUROLOGICAL DISORDERS; COMBINATION THERAPY; VIROLOGICAL MARKERS; VARYING SEVERITY; TYPE-1; INFECTION;
Keywords:
HIV-1; viral load; PCR; cultivation; cerebrospinal fluid;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
31
Recensione:
Indirizzi per estratti:
Indirizzo: Andersson, LM Gothenburg Univ, Dept Infect Dis, Inst Internal Med, Sahlgrenska Univ Hosp, S-41685 Gothenburg, Sweden Gothenburg Univ Gothenburg Sweden S-41685 thenburg, Sweden
Citazione:
L.M. Andersson et al., "Higher HIV-1 RNA cutoff level required in cerebrospinal fluid than in blood to predict positive HIV-1 isolation", J MED VIROL, 62(1), 2000, pp. 9-13

Abstract

HIV-1 can be isolated from the vast majority of blood samples taken from HIV-1-seropositive patients not treated with antiretroviral drugs. Isolationrates from cerebrospinal fluid (CSF) samples are considerably lower, ranging between 20-70%. The objective of this study was to determine the cutoff levels for HIV-I RNA that would yield a positive predictive value greater than or equal to 90% for positive virus isolation from CSF and blood. Quantitative HIV-1 RNA PCR (Amplicor HIV monitor, version 1.0, Roche Diagnostic Systems) and virus isolation were used to examine 303 CSF samples and 278 paired blood samples from 157 HIV-1-seropositive patients. Patients on antiretroviral treatment provided 140 of the CSF samples and 131 of the blood samples. CSF samples that were positive by culture numbered 137 of 303 (45%), as compared with 216 of 278 (78%) blood samples. In the case of samples taken from patients with antiretroviral treatment, 28% were positive by culture from CSF and 63% from blood. As expected, mean HIV-1 RNA levels were higher in CSF and blood samples positive by culture than in samples negative byculture. A cutoff level of >5,000 HIV-1 RNA copies/ml was required to yield a positive predictive value for positive virus isolation from CSF samplesof greater than or equal to 90%, whereas the cutoff level for blood samples was just above the detection limit of the assay (>200 HIV-1 copies/ mi). J. Med. Virol. 62:9-13, 2000. (C) 2000 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/01/20 alle ore 07:10:56