Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Authentic Borna disease virus transcripts are spliced less efficiently than cDNA-derived viral RNAs
Autore:
Jehle, C; Lipkin, WI; Staeheli, P; Marion, RM; Schwemmle, M;
Indirizzi:
Univ Freiburg, Inst Med Microbiol & Hyg, Dept Virol, D-79104 Freiburg, Germany Univ Freiburg Freiburg Germany D-79104 Virol, D-79104 Freiburg, Germany Univ Calif Irvine, Dept Neurol, Emerging Dis Lab, Irvine, CA 92697 USA Univ Calif Irvine Irvine CA USA 92697 rging Dis Lab, Irvine, CA 92697 USA Univ Calif Irvine, Dept Anat & Neurobiol, Emerging Dis Lab, Irvine, CA 92697 USA Univ Calif Irvine Irvine CA USA 92697 rging Dis Lab, Irvine, CA 92697 USA Univ Calif Irvine, Dept Microbiol & Mol Genet, Emerging Dis Lab, Irvine, CA 92697 USA Univ Calif Irvine Irvine CA USA 92697 rging Dis Lab, Irvine, CA 92697 USA CSIC, Ctr Nacl Biotecnol, E-28049 Madrid, Spain CSIC Madrid Spain E-28049 SIC, Ctr Nacl Biotecnol, E-28049 Madrid, Spain
Titolo Testata:
JOURNAL OF GENERAL VIROLOGY
, volume: 81, anno: 2000,
parte:, 8
pagine: 1947 - 1954
SICI:
0022-1317(200008)81:<1947:ABDVTA>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
MESSENGER-RNA; NUCLEAR EXPORT; NS1 PROTEIN; NUCLEOCYTOPLASMIC TRANSPORT; INFECTED-CELLS; ELEMENT; EXPRESSION; SEQUENCE; TYPE-1; IDENTIFICATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
36
Recensione:
Indirizzi per estratti:
Indirizzo: Schwemmle, M Univ Freiburg, Inst Med Microbiol & Hyg, Dept Virol, Hermann Herder Str 11, D-79104 Freiburg, Germany Univ Freiburg Hermann Herder Str 11 Freiburg Germany D-79104
Citazione:
C. Jehle et al., "Authentic Borna disease virus transcripts are spliced less efficiently than cDNA-derived viral RNAs", J GEN VIROL, 81, 2000, pp. 1947-1954

Abstract

Borna disease virus (BDV) is a non-segmented, negative-strand RNA virus that replicates and transcribes its genome in the nucleus of infected cells. It uses the cellular splicing machinery to generate a set of alternatively spliced mRNAs from the 2.8 and 7.1 kb primary transcripts, each harbouring two introns. To determine whether splicing of these transcripts is regulated by viral factors, the extent of splicing was studied in infected cells and COS-7 cells transiently transfected with plasmids encoding the 2.8 kb RNAof BDV. Unspliced RNA was found to be the most abundant RNA species in infected cells, whereas viral transcripts lacking both introns were only foundin minute amounts. In sharp contrast, plasmid-derived 2.8 kb RNA was predominantly intron 1-spliced and double-spliced. Co-expression of the BDV proteins P, N and X did not influence splicing of plasmid-expressed 2.8 kb RNA. Furthermore, the splicing pattern did not change when the 2.8 kb RNA was expressed in BDV-infected cells. Based on these results we speculate that splicing of authentic BDV transcripts is tightly linked to transcription by the viral polymerase.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 18/01/20 alle ore 13:59:38