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Titolo:
Distribution of nociceptin/orphanin FQ precursor protein and receptor in brain and spinal cord: A study using in situ hybridization and X-gal histochemistry in receptor-deficient mice
Autore:
Houtani, T; Nishi, M; Takeshima, H; Sato, K; Sakuma, S; Kakimoto, S; Ueyama, T; Noda, T; Sugimoto, T;
Indirizzi:
Kansai Med Univ, Dept Anat & Brain Sci, Moriguchi, Osaka 5708506, Japan Kansai Med Univ Moriguchi Osaka Japan 5708506 guchi, Osaka 5708506, Japan Univ Tokyo, Fac Med, Dept Pharmacol, Tokyo 1138654, Japan Univ Tokyo Tokyo Japan 1138654 Med, Dept Pharmacol, Tokyo 1138654, Japan Inst Canc, Dept Cell Biol, Tokyo 1708455, Japan Inst Canc Tokyo Japan 1708455 Canc, Dept Cell Biol, Tokyo 1708455, Japan
Titolo Testata:
JOURNAL OF COMPARATIVE NEUROLOGY
fascicolo: 3, volume: 424, anno: 2000,
pagine: 489 - 508
SICI:
0021-9967(20000828)424:3<489:DONFPP>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
LONG-TERM POTENTIATION; ORPHAN OPIOID RECEPTOR; CENTRAL-NERVOUS-SYSTEM; CA2+ CHANNEL CURRENT; NEURONS IN-VITRO; TISSUE DISTRIBUTION; ORL(1) RECEPTOR; MOLECULAR-CLONING; ENDOGENOUS LIGAND; GENE FAMILY;
Keywords:
beta-galactosidase; gene targeting; peptide receptor system; knockout mice; hearing; learning and memory;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
64
Recensione:
Indirizzi per estratti:
Indirizzo: Sugimoto, T Kansai Med Univ, Dept Anat & Brain Sci, Moriguchi, Osaka 5708506, Japan Kansai Med Univ Moriguchi Osaka Japan 5708506 5708506, Japan
Citazione:
T. Houtani et al., "Distribution of nociceptin/orphanin FQ precursor protein and receptor in brain and spinal cord: A study using in situ hybridization and X-gal histochemistry in receptor-deficient mice", J COMP NEUR, 424(3), 2000, pp. 489-508

Abstract

Nociceptin/orphanin FQ (N/OFQ) is an opioid-like heptadecapeptide agonist for the opioid receptor homolog, N/OFQ receptor. To explore the precise distribution of the peptide-receptor system, the authors examined the brain and spinal cord from receptor-deficient mice bearing the targeted mutation (morc(m1)), a LacZ insertional mutation in the N/OFQ receptor gene. Precursorprotein N/OFQ (preproN/OFQ) mRNA was detected by using in situ hybridization, and the N/OFQ receptor was detected by using X-gal histochemistry. The N/OFQ receptor reflected by lacZ expression was observed at high levels in the dentate gyrus, lateral septum, subparafascicular thalamic nucleus, medial preoptic area, median preoptic nucleus, ventromedial preoptic nucleus, anterior hypothalamic area, paraventricular hypothalamic nucleus, ventromedial hypothalamic nucleus, auditory brainstem nuclei, pontine dorsal tegmentum, and nucleus of the solitary tract. In situ detection of the N/OFQ receptor mRNA by digoxigenin-labeled riboprobes coupled with tyramide signal amplification in normal and wild-type mice resulted in the regional distribution paralleling the lacZ expression in these regions. PreproN/OFQ mRNA was expressed at high levels in the subparafascicular thalamic nucleus, central gray, central tegmental field, auditory brainstem nuclei, caudal spinal trigeminal nucleus, and spinal dorsal hem. Furthermore, variable levels of expression of the peptide and receptor were seen in distinct sites of the brainand spinal cord. These data indicate a correspondence of the peptide and the receptor in local distribution at limbic, hypothalamic, and brainstem sites. Together with concurrent physiologic and behavioral studies in mutant mice, the results suggest functional roles for the N/OFQ system, including the central regulation of learning and memory, hearing ability, water balance, food intake, and blood pressure. (C) 2000 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/07/20 alle ore 21:29:12