Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Rad51 accumulation at sites of DNA damage and in postreplicative chromatin
Autore:
Tashiro, S; Walter, J; Shinohara, A; Kamada, N; Cremer, T;
Indirizzi:
Hiroshima Univ, Fac Med, Dept Pediat, Minami Ku, Hiroshima 7348551, Japan Hiroshima Univ Hiroshima Japan 7348551 nami Ku, Hiroshima 7348551, Japan Univ Munich, Inst Anthropol & Human Genet, D-80333 Munich, Germany Univ Munich Munich Germany D-80333 Human Genet, D-80333 Munich, Germany Hiroshima Univ, Res Inst Radiat Biol & Med, Hiroshima 7348551, Japan Hiroshima Univ Hiroshima Japan 7348551 l & Med, Hiroshima 7348551, Japan Univ Chicago, Dept Radiat & Cellular Oncol, Chicago, IL 60637 USA Univ Chicago Chicago IL USA 60637 & Cellular Oncol, Chicago, IL 60637 USA Hiroshima Univ, Fac Med, Dept Pediat, Hiroshima, Japan Hiroshima Univ Hiroshima Japan , Fac Med, Dept Pediat, Hiroshima, Japan
Titolo Testata:
JOURNAL OF CELL BIOLOGY
fascicolo: 2, volume: 150, anno: 2000,
pagine: 283 - 291
SICI:
0021-9525(20000724)150:2<283:RAASOD>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
DOUBLE-STRAND BREAKS; NUCLEAR FOCI; CHROMOSOME TERRITORIES; RECOMBINATION PROTEINS; ULTRAVIOLET-LIGHT; CELLS; REPAIR; RECA; LOCALIZATION; LYMPHOCYTES;
Keywords:
Rad51; DNA damage; microirradiation; postreplicative DNA repair; indirect immunofluorescence;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
38
Recensione:
Indirizzi per estratti:
Indirizzo: Tashiro, S Hiroshima Univ, Fac Med, Dept Pediat, Minami Ku, Kasumi 1-2-3, Hiroshima 7348551, Japan Hiroshima Univ Kasumi 1-2-3 Hiroshima Japan 7348551 551, Japan
Citazione:
S. Tashiro et al., "Rad51 accumulation at sites of DNA damage and in postreplicative chromatin", J CELL BIOL, 150(2), 2000, pp. 283-291

Abstract

Rad51, a eukaryotic RecA homologue, plays a central role in homologous recombinational repair of DNA double-strand breaks (DSBs) in yeast and is conserved from yeast to human. Rad51 shows punctuate nuclear localization in human cells, called Rad51 foci, typically during the S phase (Tashiro, S., N. Kotomura, A. Shinohara, K. Tanaka, K. Ueda, and N. Kamada. 1996. Oncogene.12:2165-2170). However, the topological relationships that exist in human S phase nuclei between Rad51 foci and damaged chromatin have not been studied thus far. Here, we report on ultraviolet microirradiation experiments ofsmall nuclear areas and on whole cell ultraviolet C (UVC) irradiation experiments performed with a human fibroblast cell line. Before UV irradiation,nuclear DNA was sensitized by the incorporation of halogenated thymidine analogues. These experiments demonstrate the redistribution of Rad51 to the selectively damaged, labeled chromatin. Rad51 recruitment takes place from Rad51 foci scattered throughout the nucleus of nonirradiated cells in S phase. We also demonstrate the preferential association of Rad51 foci with postreplicative chromatin in contrast to replicating chromatin using a double labeling procedure with halogenated thymidine analogues,This finding supports a role of Rad51 in recombinational repair processes of DNA damage present in postreplicative chromatin.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/10/20 alle ore 14:53:05