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Titolo:
A direct interaction between proliferating cell nuclear antigen (PCNA) andCdk2 targets PCNA-interacting proteins for phosphorylation
Autore:
Koundrioukoff, S; Jonsson, ZO; Hasan, S; de Jong, RN; van der Vliet, PC; Hottiger, MO; Hubscher, U;
Indirizzi:
Univ Zurich Irchel, Inst Vet Biochem, CH-8057 Zurich, Switzerland Univ Zurich Irchel Zurich Switzerland CH-8057 H-8057 Zurich, Switzerland Univ Utrecht, Physiol Chem Lab, NL-3584 CG Utrecht, Netherlands Univ Utrecht Utrecht Netherlands NL-3584 CG 3584 CG Utrecht, Netherlands Ctr Biomed Genet, NL-3584 CG Utrecht, Netherlands Ctr Biomed Genet Utrecht Netherlands NL-3584 CG CG Utrecht, Netherlands
Titolo Testata:
JOURNAL OF BIOLOGICAL CHEMISTRY
fascicolo: 30, volume: 275, anno: 2000,
pagine: 22882 - 22887
SICI:
0021-9258(20000728)275:30<22882:ADIBPC>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
DEPENDENT KINASE INHIBITOR; DNA-REPLICATION; BINDING-PROTEIN; REPAIR; SUBUNIT; CYCLINS; FEN-1; ALPHA; P21;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
27
Recensione:
Indirizzi per estratti:
Indirizzo: Hubscher, U Univ Zurich Irchel, Inst Vet Biochem, Winterthurerstr 190, CH-8057 Zurich,Switzerland Univ Zurich Irchel Winterthurerstr 190 Zurich Switzerland CH-8057
Citazione:
S. Koundrioukoff et al., "A direct interaction between proliferating cell nuclear antigen (PCNA) andCdk2 targets PCNA-interacting proteins for phosphorylation", J BIOL CHEM, 275(30), 2000, pp. 22882-22887

Abstract

Proliferating cell nuclear antigen is best known as a DNA polymerase accessory protein but has more recently also been shown to have different functions in important cellular processes such as DNA replication, DNA repair, and cell cycle control. PCNA has been found in quaternary complexes with the cyclin kinase inhibitor p21 and several pairs of cyclin-dependent protein kinases and their regulatory partner, the cyclins. Here we show a direct interaction between PCNA and Cdk2. This interaction involves the regions of the PCNA trimer close to the C termini. We found that PCNA and Cdk2 form a complex together with cyclin A. This ternary PCNA-Cdk2-cyclin A complex was able to phosphorylate the PCNA binding region of the large subunit of replication factor C as well as DNA ligase I. Furthermore, PCNA appears to be a connector between Cdk2 and DNA ligase I and to stimulate phosphorylation of DNA ligase I. Based on our results, we propose the model that PCNA brings Cdk2 to proteins involved in DNA replication and possibly might act as an "adaptor" for Cdk2-cyclin A to PCNA-binding DNA replication proteins.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/09/20 alle ore 16:57:18