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Titolo:
Glutathione limits noise-induced hearing loss
Autore:
Ohinata, Y; Yamasoba, T; Schacht, J; Miller, JM;
Indirizzi:
Univ Michigan, Kresge Hearing Res Inst, Ann Arbor, MI 48109 USA Univ Michigan Ann Arbor MI USA 48109 ng Res Inst, Ann Arbor, MI 48109 USA Osaka Med Coll, Dept Otolaryngol, Osaka 5698686, Japan Osaka Med Coll Osaka Japan 5698686 ept Otolaryngol, Osaka 5698686, Japan Univ Tokyo, Dept Otolaryngol, Bunkyo Ku, Tokyo 1138655, Japan Univ Tokyo Tokyo Japan 1138655 laryngol, Bunkyo Ku, Tokyo 1138655, Japan
Titolo Testata:
HEARING RESEARCH
fascicolo: 1-2, volume: 146, anno: 2000,
pagine: 28 - 34
SICI:
0378-5955(200008)146:1-2<28:GLNHL>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
COCHLEAR BLOOD-FLOW; LASER DOPPLER MEASUREMENTS; PIG INNER-EAR; GUINEA-PIG; LOUD SOUND; GENTAMICIN OTOTOXICITY; CISPLATIN OTOTOXICITY; ACOUSTIC TRAUMA; CELL VELOCITY; FREE-RADICALS;
Keywords:
reactive oxygen species; antioxidant; noise; hearing loss; diet; guinea pig;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
45
Recensione:
Indirizzi per estratti:
Indirizzo: Miller, JM Univ Michigan, Kresge Hearing Res Inst, 1301 E Ann St, Ann Arbor, MI 48109USA Univ Michigan 1301 E Ann St Ann Arbor MI USA 48109 MI 48109USA
Citazione:
Y. Ohinata et al., "Glutathione limits noise-induced hearing loss", HEARING RES, 146(1-2), 2000, pp. 28-34

Abstract

The generation of reactive oxygen species (ROS) is thought to be part of the mechanism underlying noise-induced hearing loss (NIHL). Glutathione (GSH) is an important cellular antioxidant that limits cell damage by ROS. In this study, we investigated the effectiveness of a GSH supplement to protectGSH-deficient animals from NIHL. Pigmented guinea pigs were exposed to a 4kHz octave band noise, 115 dB SPL, for 5 h. Group 1 had a normal diet, while groups 2, 3 and 4 were fed a 7% low protein diet (leading to lowered tissue levels of GSH) for 10 days prior to noise exposure. One hour before, immediately after and 5 h after noise exposure, subjects received either an intraperitoneal injection of 5 ml/kg body weight of 0.9% NaCl (groups 1 and 2), 0.4 M glutathione monoethyl eater (GSHE; group 3) or 0.8 M GSHE (group 4). Auditory thresholds were measured by evoked brain stem response at 2, 4, 8, 12, 16 and 20 kHz before and after noise exposure. Ten days post exposure, group 1 showed noise-induced threshold shifts of approximately 20 dB at 2, 16 and 20 kHz and 35 to 40 dB at other frequencies. Threshold shifts in group 2 were significantly greater than baseline at 2, 4, 16 and 20 kHz. GSHE supplementation in a dose-dependent fashion attenuated the threshold shifts in the low protein diet animals. Hair cell loss, as evaluated with cytocochleograms, was consistent with the auditory-evoked brainstem response results. Group 2 exhibited significantly more hair cell loss than any of the other groups; hair cell loss in group 3 was similar to that seen in group1; group 4 showed less loss than group 1. These results indicate that GSH is a significant factor in limiting noise-induced cochlear damage. This is compatible with the notion that ROS generation plays a role in NIHL and that antioxidant treatment may be an effective prophylactic intervention. (C) 2000 Elsevier Science B.V. All rights reserved.

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Documento generato il 22/09/20 alle ore 07:26:43