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Titolo:
Establishing neuronal identity in vertebrate neurogenic placodes
Autore:
Baker, CVH; Bronner-Fraser, M;
Indirizzi:
CALTECH, Beckman Inst 139 74, Div Biol, Pasadena, CA 91125 USA CALTECH Pasadena CA USA 91125 st 139 74, Div Biol, Pasadena, CA 91125 USA
Titolo Testata:
DEVELOPMENT
fascicolo: 14, volume: 127, anno: 2000,
pagine: 3045 - 3056
SICI:
0950-1991(200007)127:14<3045:ENIIVN>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
NEURAL CREST CELLS; HOX 2.1 PROTEIN; MONOCLONAL-ANTIBODIES; ECTODERMAL PLACODES; HOMEODOMAIN PROTEIN; TRIGEMINAL PLACODE; SENSORY NEURONS; NERVOUS-SYSTEM; CHICK-EMBRYO; PAX GENES;
Keywords:
placodes; trigeminal placode; nodose placode; Pax2; Pax3; Phox2a; chick; quail; sensory neurons;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
59
Recensione:
Indirizzi per estratti:
Indirizzo: Baker, CVH CALTECH, Beckman Inst 139 74, Div Biol, Pasadena, CA 91125 USA CALTECH Pasadena CA USA 91125 Div Biol, Pasadena, CA 91125 USA
Citazione:
C.V.H. Baker e M. Bronner-Fraser, "Establishing neuronal identity in vertebrate neurogenic placodes", DEVELOPMENT, 127(14), 2000, pp. 3045-3056

Abstract

The trigeminal and epibranchial placodes of vertebrate embryos form different types of sensory neurons. The trigeminal placodes form cutaneous sensory neurons that innervate the face and jaws, while the epibranchial placodes(geniculate, petrosal and nodose) form visceral sensory neurons that innervate taste buds and visceral organs. In the chick embryo, the ophthalmic trigeminal (opV) placode expresses the paired homeodomain transcription factor Pax3 from every early stages, while the epibranchial placodes express Pax2. Here, we show that Pax3 expression in explanted opV placode ectoderm correlates at the single cell level with neuronal specification and with commitment to an opV fate. When opV (trigeminal) ectoderm is grafted in place ofthe nodose (epibranchial) placode, Pax3-expressing cells form Pax3-positive neurons on the same schedule as in the opV placode. In contrast, Pax3-negative cells in the grafted ectoderm are induced to express the epibranchialplacode marker Pax2. and form neurons in the nodose ganglion that express the epibranchial neuron marker Phox2a on the same schedule as host nodose neurons. They also project neurites along central and peripheral nodose neurite pathways and survive until well after the main period of cell death in the nodose ganglion. The older the opV ectoderm is at the time of grafting,the more Pax3-positive cells it contains and the more committed it is to an opV fate. Our results suggest that, within the neurogenic placodes, theredoes not appear to be a two-step induction of 'generic' neurons followed by specification of the neuron to a particular fate. Instead, there seems tobe a one-step induction in which neuronal subtype identity is coupled to neuronal differentiation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/03/20 alle ore 19:08:35