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Titolo:
Elevated protein content and prolyl 4-hydroxylase activity in severely degenerated human annulus fibrosus
Autore:
Kaapa, E; Wang, W; Takala, TES; Poussa, M; Konttinen, YT; Lammi, MJ; Vanharanta, H;
Indirizzi:
Univ Kuopio, Dept Anat, Kuopio 70211, Finland Univ Kuopio Kuopio Finland70211 uopio, Dept Anat, Kuopio 70211, Finland Univ Helsinki Hosp, Dept Phys Med & Rehabil, Helsinki, Finland Univ Helsinki Hosp Helsinki Finland ys Med & Rehabil, Helsinki, Finland Univ Helsinki Hosp, Dept Med 4, Helsinki, Finland Univ Helsinki Hosp Helsinki Finland Hosp, Dept Med 4, Helsinki, Finland Univ Jyvaskyla, Dept Biol Phys Act, SF-40351 Jyvaskyla, Finland Univ Jyvaskyla Jyvaskyla Finland SF-40351 t, SF-40351 Jyvaskyla, Finland Invalid Fdn, Orthopaed Hosp, Helsinki, Finland Invalid Fdn Helsinki Finland lid Fdn, Orthopaed Hosp, Helsinki, Finland Univ Helsinki, Inst Biomed, Dept Anat, FIN-00014 Helsinki, Finland Univ Helsinki Helsinki Finland FIN-00014 at, FIN-00014 Helsinki, Finland Oulu Univ, Dept Phys Med & Rehabil, Oulu, Finland Oulu Univ Oulu Finland ulu Univ, Dept Phys Med & Rehabil, Oulu, Finland
Titolo Testata:
CONNECTIVE TISSUE RESEARCH
fascicolo: 2, volume: 41, anno: 2000,
pagine: 93 - 99
SICI:
0300-8207(2000)41:2<93:EPCAP4>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
LUMBAR INTERVERTEBRAL DISC; PERFORMANCE LIQUID-CHROMATOGRAPHY; ARTICULAR-CARTILAGE; COLLAGEN-METABOLISM; MUSCLE; AGE; IMMUNOLOCALIZATION; DENATURATION; HYPERTROPHY; CROSSLINKS;
Keywords:
collagen biosynthesis; collagen crosslinks; disc degeneration; prolyl 4-hydroxylase;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
46
Recensione:
Indirizzi per estratti:
Indirizzo: Lammi, MJ Univ Kuopio, Dept Anat, POB 1627, Kuopio 70211, Finland Univ Kuopio POB 1627 Kuopio Finland 70211 Kuopio 70211, Finland
Citazione:
E. Kaapa et al., "Elevated protein content and prolyl 4-hydroxylase activity in severely degenerated human annulus fibrosus", CONNECT TIS, 41(2), 2000, pp. 93-99

Abstract

Alterations involved with the intervertebral disc degeneration are partly well described, however, it is not so well known how collagen network is affected by the disease. We analyzed the rate of collagen biosynthesis (estimated by the enzymic activities of prolyl 4-hydroxylase and galactosylhydroxylysyl glucosyltransferase) and the level of hydroxylysylpyridinoline and lysylpyridinoline crosslinks both in normal(n=7) and degenerated (n=7) humanannulus fibrosus, The activity of prolyl 4-hydroxylase was significantly increased in degenerated tissue. However, no significant changes in the collagen content or in the amount of hydroxylysylpyridinoline and lysylpyridinoline collagen crosslinks were observed, On the other hand, the content of soluble proteins was significantly increased. Our results suggest that collagen biosynthesis is increased in degenerated human annulus fibrosus, obviously to compensate the impairment of collagen fibers, The faster turnover ofcollagen in degenerated annulus fibrosus, suggested by the increased prolyl-4-hydroxylase activity and unchanged collagen content, seems not to causeany significant changes in its mature pyridinium crosslink concentrations.

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Documento generato il 28/09/20 alle ore 11:11:05