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Titolo:
Two-color double-labeling in situ hybridization of whole-mount Hydra usingRNA probes for five different Hydra neuropeptide preprohormones: evidence for colocalization
Autore:
Hansen, GN; Williamson, M; Grimmelikhuijzen, CJP;
Indirizzi:
Univ Copenhagen, Inst Zool, Dept Cell Biol, DK-2100 Copenhagen O, Denmark Univ Copenhagen Copenhagen Denmark O Biol, DK-2100 Copenhagen O, Denmark
Titolo Testata:
CELL AND TISSUE RESEARCH
fascicolo: 2, volume: 301, anno: 2000,
pagine: 245 - 253
SICI:
0302-766X(200008)301:2<245:TDISHO>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
SEA-ANEMONE NEUROPEPTIDE; LESS-THAN GLU-GLY-ARG-PHE-NH2; SENSORY-MOTOR-INTERNEURON; ANTHO-RWAMIDE-II; NERVOUS-SYSTEM; ULTRASTRUCTURAL-LOCALIZATION; CALLIACTIS-PARASITICA; MOLECULAR-CLONING; MESSENGER-RNA; RFAMIDE;
Keywords:
neurohormone; coexpression; neuron; nervous system; Hydra magnipapillata (Cnidaria);
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
38
Recensione:
Indirizzi per estratti:
Indirizzo: Grimmelikhuijzen, CJP Univ Copenhagen, Inst Zool, Dept Cell Biol, Univ Pk 15, DK-2100 CopenhagenO, Denmark Univ Copenhagen Univ Pk 15 Copenhagen Denmark O ark
Citazione:
G.N. Hansen et al., "Two-color double-labeling in situ hybridization of whole-mount Hydra usingRNA probes for five different Hydra neuropeptide preprohormones: evidence for colocalization", CELL TIS RE, 301(2), 2000, pp. 245-253

Abstract

The freshwater polyp Hydra magnipapillata has a primitive nervous system that produces at least. three distinct classes of neuropeptides: various peptides having the C-terminal sequence Arg-Phe-NH2 (the Hydra-RFamide family), Leu-Trp-NH2 (the Hydra-LWamide family), and a single peptide having the C-terminal sequence Lys-Val-NH2 (Hydra-KVamide). The various Hydra-RFamides are synthesized by three different preprohormones: preprohormone-A, -B, and-C. The various Hydra-LWamides are synthesized by a single preprohormone (prepro-Hydra-LWamide), as is Hydra-KVamide (prepro-Hydra-KVamide). Using a wholemount double-labeling two-color in situ hybridization technique and RNA probes specific for each of these five Hydra preprohormone mRNAs, we found that specific sets of neurons express each of the five preprohormones, except for the peduncle region of Hydra tan area just above the basal disk), where a population of neurons exists that expresses both preprohormones-A and prepro-Hydra-KVamide mRNAs. The functional significance of this coexpression is unclear. This is the first report on the coexpression of two well-characterized preprohormones (yielding two well-characterized neurohormone families) in cnidarians. This report also shows that there are at least six neurochemically different populations of neurons in Hydra.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 15:06:57