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Titolo:
Expression and secretion of scytalidopepsin B, an acid protease from Scytalidium lignicolum, in yeast
Autore:
Shimuta, K; Oda-Ueda, N; Washio, M; Oyama, H; Oda, K; Tsuru, D;
Indirizzi:
Sojo Univ, Dept Appl Microbial Technol, Kumamoto 8600082, Japan Sojo UnivKumamoto Japan 8600082 robial Technol, Kumamoto 8600082, Japan Kyoto Inst Technol, Fac Text Sci, Sakyo Ku, Kyoto 6060962, Japan Kyoto Inst Technol Kyoto Japan 6060962 i, Sakyo Ku, Kyoto 6060962, Japan
Titolo Testata:
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
fascicolo: 7, volume: 64, anno: 2000,
pagine: 1542 - 1546
SICI:
0916-8451(200007)64:7<1542:EASOSB>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
TRANSFORMATION; SEQUENCE; GENE;
Keywords:
pepstatin-insensitive acid protease; scytalidopepsin B; yeast invertase signal segment; shuttle vector;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
12
Recensione:
Indirizzi per estratti:
Indirizzo: Tsuru, D Sojo Univ, Dept Appl Microbial Technol, Ikeda 4-22-1, Kumamoto 8600082, Japan Sojo Univ Ikeda 4-22-1 Kumamoto Japan 8600082 oto 8600082, Japan
Citazione:
K. Shimuta et al., "Expression and secretion of scytalidopepsin B, an acid protease from Scytalidium lignicolum, in yeast", BIOS BIOT B, 64(7), 2000, pp. 1542-1546

Abstract

An expression and secretion system for scytalidopepsin B, an acid proteasefrom Scytalidium lignicolum, was constructed in yeast. Saccharomyces cerevisiae AH22 was transformed with an yeast-E. coli shuttle vector, pAM82, in which an yeast invertase signal segment and the cDNA encoding the pro- and mature enzyme regions were inserted. The transformant was found to secret apepstatin-insensitive acid protease, when cultured aerobically in a low phosphate (Pi) medium. Amino terminal amino acid sequencing analysis indicated that the recombinant acid protease was accurately processed and secreted as a mature form.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/01/20 alle ore 20:20:26