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Titolo:
Expression of YAV proteins and vaccination against viral ascites among cultured juvenile yellowtail
Autore:
Sato, H; Nakajima, K; Maeno, Y; Kamaishi, T; Kamata, T; Mori, H; Kamei, K; Takano, R; Kudo, KI; Hara, S;
Indirizzi:
Kyoto Inst Technol, Dept Appl Biol, Kyoto 6068585, Japan Kyoto Inst Technol Kyoto Japan 6068585 t Appl Biol, Kyoto 6068585, Japan Sanwa Cornstarch Co Ltd, Nara 6308585, Japan Sanwa Cornstarch Co Ltd Nara Japan 6308585 h Co Ltd, Nara 6308585, Japan
Titolo Testata:
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
fascicolo: 7, volume: 64, anno: 2000,
pagine: 1494 - 1499
SICI:
0916-8451(200007)64:7<1494:EOYPAV>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
PANCREATIC NECROSIS VIRUS; RAINBOW-TROUT; PROTECTIVE IMMUNITY; ESCHERICHIA-COLI; SEGMENT-A; POLYPROTEIN; EPITOPE; GLYCOPROTEIN; VECTORS; MYKISS;
Keywords:
YAV; segment A; baculovirus; gene expression; vaccination;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
23
Recensione:
Indirizzi per estratti:
Indirizzo: Hara, S Kyoto Inst Technol, Dept Appl Biol, Kyoto 6068585, Japan Kyoto Inst Technol Kyoto Japan 6068585 iol, Kyoto 6068585, Japan
Citazione:
H. Sato et al., "Expression of YAV proteins and vaccination against viral ascites among cultured juvenile yellowtail", BIOS BIOT B, 64(7), 2000, pp. 1494-1499

Abstract

Yellowtail ascites virus (YAV) is a member of the family Birnaviridae and causes viral ascites among juvenile yellowtail (Seriola quinqueradiata). Wehave reported the cloning and expression of two viral cDNAs, the first being segment A encoding a polyprotein of viral capsid proteins (VP2 and VP3) and a protease (NS), and the second being VP2-epitope encoding serotype-specific epitope region on VP2, using a baculovirus expression system. Anotherviral cDNA encoding a polyprotein of NS and VP3 was cloned and expressed in this study. For the expression of NS/VP3 (YAV nt 1626 to 3066) in insect cells a 31-kDa protein, corresponding to VP3 was detected, indicating an appropriate posttranslational processing of NS/VP3 polypeptide by NS proteaseitself. The analysis of the N-terminal amino acid sequence of this proteinshowed that NS protease may cleave an Ala-Ser bond. A study of the potential for vaccination of yellowtail fry by injection of insect cell lysates infected with baculovirus, containing either cDNA of segment A, VP2-epitope, or NS/ VP3 was undertaken. Only a vaccination with cell lysates infected with a recombinant virus carrying the full length of YAV segment A gene demonstrated approximately the same effect as that of inactivated YAV. This result suggested that all proteins VP2, VP3, and NS are required for an effective vaccination.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/03/20 alle ore 23:29:37