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Titolo:
Colloidal properties of human transferrin receptor in detergent free solution
Autore:
Schuler, J; Frank, J; Behlke, J; Saenger, W; Georgalis, Y;
Indirizzi:
Free Univ Berlin, Inst Kristallog, D-14195 Berlin, Germany Free Univ Berlin Berlin Germany D-14195 stallog, D-14195 Berlin, Germany Max Planck Gesell, Fritz Haber Inst, D-14195 Berlin, Germany Max Planck Gesell Berlin Germany D-14195 r Inst, D-14195 Berlin, Germany Max Delbruck Ctr Mol Med, D-13122 Berlin, Germany Max Delbruck Ctr Mol Med Berlin Germany D-13122 D-13122 Berlin, Germany
Titolo Testata:
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY
fascicolo: 1-2, volume: 1480, anno: 2000,
pagine: 211 - 221
SICI:
0167-4838(20000714)1480:1-2<211:CPOHTR>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
LIGHT-SCATTERING; EXTRACELLULAR FRAGMENT; INTEGRAL-EQUATIONS; PROTEINS; OLIGOSACCHARIDES; AGGREGATION; STABILITY; MEMBRANE; FRACTALS; PROGRAM;
Keywords:
static light scattering; photon correlation spectroscopy; fractal aggregate; analytical ultracentrifugation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
41
Recensione:
Indirizzi per estratti:
Indirizzo: Saenger, W Free Univ Berlin, Inst Kristallog, Takustr 6, D-14195 Berlin, Germany Free Univ Berlin Takustr 6 Berlin Germany D-14195 lin, Germany
Citazione:
J. Schuler et al., "Colloidal properties of human transferrin receptor in detergent free solution", BBA-PROT ST, 1480(1-2), 2000, pp. 211-221

Abstract

The colloidal properties of transferrin receptor, isolated from human placenta, in detergent free solution has been investigated by light scattering techniques and analytical ultracentrifugation. In detergent free solution at 293.2 K, hTfR forms stable aggregates with an apparent hydrodynamic radius of 17 nm. The molecular mass was determined by ultracentrifugation to liebetween (1722 +/- 87) kDa (sedimentation equilibrium) and (1675 +/- 46) kDa (sedimentation velocity). This implies that the aggregates are build up from nine hTfR dimers. Based on model calculations, which are in good agreement with the experimental data, we propose a torus-like structure for the aggregates. Upon pH shift from pH 7.5 to 5.0 or removal of the N-linked carbohydrate chains, formation of larger aggregates is induced. These aggregates can be described in terms of porous fractal structures. We propose a simple model, which accounts for that behaviour assuming that the aggregation is mainly due to the reduction of negative surface charge. (C) 2000 ElsevierScience B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 15:03:06