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Titolo:
ALTERATION IN THE CONTROL OF MITOCHONDRIAL RESPIRATION BY OUTER MITOCHONDRIAL-MEMBRANE AND CREATINE DURING HEART PRESERVATION
Autore:
KAY L; DANESHRAD Z; SAKS VA; ROSSI A;
Indirizzi:
UNIV GRENOBLE 1,LAB BIOENERGET,BP 53 F-38041 GRENOBLE 9 FRANCE UNIV GRENOBLE 1,LAB BIOENERGET F-38041 GRENOBLE 9 FRANCE
Titolo Testata:
Cardiovascular Research
fascicolo: 3, volume: 34, anno: 1997,
pagine: 547 - 556
SICI:
0008-6363(1997)34:3<547:AITCOM>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
EARLY CONTRACTILE FAILURE; HIGH-ENERGY PHOSPHATES; OXIDATIVE-PHOSPHORYLATION; MYOCARDIAL-ISCHEMIA; INORGANIC-PHOSPHATE; RAT-HEART; CELLULAR RESPIRATION; CARDIAC FIBERS; IN-VIVO; KINASE;
Keywords:
HEART PRESERVATION; MYOCARDIAL ISCHEMIA; SKINNED FIBERS; MITOCHONDRIA, OUTER MEMBRANE; CREATINE KINASE; RAT, HEART;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
40
Recensione:
Indirizzi per estratti:
Citazione:
L. Kay et al., "ALTERATION IN THE CONTROL OF MITOCHONDRIAL RESPIRATION BY OUTER MITOCHONDRIAL-MEMBRANE AND CREATINE DURING HEART PRESERVATION", Cardiovascular Research, 34(3), 1997, pp. 547-556

Abstract

Objective: This study aimed to evaluate the nature and extent of mitochondrial alterations during heart preservation. Methods: Rat hearts, isolated after cardioplegia in situ, were preserved for 6 or 15 h at 4degrees C either by immersion in cardioplegic solution or by low-flowperfusion (0.3 ml/min) with cardioplegic solution. The energy state of hearts at the end of preservation was determined by P-31-NMR spectroscopy and functional recovery was evaluated on reperfusion. Variables of mitochondrial respiration (maximal rate of respiration in the presence of ADP = DDVmax, apparent K-m for ADP, effect of creatine) were evaluated on skinned fibers and compared with those determined in controls and in hearts subjected to 1 hour of ischemia at 37 degrees C. Results: Serious mitochondrial alterations were detected in fibers from 15h immersed hearts: decrease of Delta V-max and of apparent K-m for ADP, loss of the stimulatory effect of creatine, and disruption of the outer mitochondrial membrane. The extent of alterations was more accentuated in fibers from normothermic ischemic hearts, in which some damage of the inner mitochondrial membrane also occured. In fibers from hearts preserved for 6 h, no significant changes in mitochondrial variables could be detected. When the hearts were preserved under low-flow perfusion for 15 h, only the stimulatory effect of creatine on respiration was significantly decreased. The extent of the loss of the stimulatory effect of creatine paralleled the accumulation of inorganic phosphate (P-i) during preservation and the decrease in left ventricular function on reperfusion. Conclusions: Alterations related to the outer membrane and the intermembrane space are among the earliest signs of damage to mitochondrial function during heart preservation. These alterations could be attributed to the swelling of mitochondria under the effect of P-i. The determination of mitochondrial parameters in biopsy samples could allow a simple and rapid evaluation of energy-producing and transfer capacities of the myocardium.

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Documento generato il 05/12/20 alle ore 20:18:21