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Titolo:
A small structural element, Pc-J5/5a, plays dual roles in a group IC1 intron RNA
Autore:
Ikawa, Y; Shiraishi, H; Inoue, T;
Indirizzi:
Kyoto Univ, Grad Sch Biostudies, Kyoto 6068502, Japan Kyoto Univ Kyoto Japan 6068502 Grad Sch Biostudies, Kyoto 6068502, Japan
Titolo Testata:
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
fascicolo: 1, volume: 274, anno: 2000,
pagine: 259 - 265
SICI:
0006-291X(20000721)274:1<259:ASSEPP>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
GROUP-I INTRON; TETRAHYMENA RIBOZYME; TERTIARY STRUCTURE; DOMAIN; SECONDARY; TETRALOOP; MOTIF; P5ABC;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
22
Recensione:
Indirizzi per estratti:
Indirizzo: Inoue, T Kyoto Univ, Grad Sch Biostudies, Kyoto 6068502, Japan Kyoto UnivKyoto Japan 6068502 Biostudies, Kyoto 6068502, Japan
Citazione:
Y. Ikawa et al., "A small structural element, Pc-J5/5a, plays dual roles in a group IC1 intron RNA", BIOC BIOP R, 274(1), 2000, pp. 259-265

Abstract

The P4-P6 domain of group IC1 intron ribozymes such as that of the Tetrahymena autonomously folds into a hairpin-shaped structure in which the J5/5a region serves as a hinge. Phylogenetic comparisons of these IC1 introns suggested that the J5/5a region (termed Pc-J5/5a motif) in a subclass of IC1 introns such as the one from Pneumocystis carinii functions not only as a hinge but also as a receptor for a GAAA-tetraloop. We investigated the role of this motif by transplanting the structural unit, Pc-J5/5a motif, of Pneumocystis carinii into the P4-P6 domain of the Tetrahymena intron. The results showed that the Pc-J5/5a motif binds to a GAAA loop with high affinity and also facilitates the bending of the Tetrahymena P4-P6 domain more positively than the original J5/5a region. (C) 2000 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/01/20 alle ore 16:06:43